Whitley M35 Workstation – A microaerobic environment for your microaerophiles

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The Whitley M35 is a microaerobic workstation ideal for the isolation and study of Campylobacter spp, Helicobacter pylori and other similarly fastidious organisms. The M35 provides a spacious working environment accommodating up to 600 x 90mm Petri dishes with a rapid 60 second airlock cycle. This is a 4-gas system with built-in gas sensing technology that allows you to programme precise gas concentrations, meaning costs can be cheaper than when using a pre mixed cylinder. Temperature and humidity can be easily controlled using the touch screen interface and a unique hydrogen sensor which offers real time feedback. This all ensures the workstation conditions are perfect for manipulating samples in a sustainable microaerobic environment.

 

 

Features and Benefits include:

  • A rapid, 12 litre airlock that can transfer 40 plates in only 60 seconds.
  • For total flexibility, up to four gases – nitrogen, carbon dioxide, air and a 10% hydrogen/90% nitrogen mix – can be combined within safe and varying ratios to provide a specific atmosphere for your experiments.
  • This unit can accommodate up to 600 x 90mm Petri dishes.
  • Intuitive colour touch screen interface with PIN-code protected user access levels.
  • Data download facility that allows you to record workstation parameters and download stored data in seconds for traceability and reference.

And a comprehensive range of other options and accessories to tailor the workstation to your specific requirements.

 

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Request a demo today by emailing info@meintrup-dws.de or complete our online contact form

Whitley H35 Hypoxystation used in heart regeneration project

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Dr. Vaibhao Janbandhu is a Postdoctoral Research Scientist at the Victor Chang Cardiac Research Institute (VCCR) in Sydney. He has been in contact with Don Whitley Scientific to explain how his lab’s work has benefited from the use of a Whitley H35 Hypoxystation. Vaibhao uses the Hypoxystation to isolate, culture and characterise adult cardiac stem cells (CSCs).

 

Dr. Janbandhu had already been using a H35 that was set up at the institute for almost three years before he got his own unit installed last year. Specifically, his project is to find new ways to stimulate heart regeneration during ageing and after heart attack. For this he needs a way to isolate, culture and characterise adult CSCs. In Vaibhao’s words the H35 Hypoxystation seems well suited for this application: “the DWS Hypoxystation provides a highly stabilised HEPA-filtered environment in which levels of oxygen, carbon dioxide, temperature and humidity are precisely controlled and it will be an integral part of the project to advance the project aims”.

 

Vaibhao explains that mammalian stem cells reside in a specialised cellular microenvironment. This niche and the stem cell niche is characterised by a low partial oxygen pressure. This hypoxic niche protects stem cells from deleterious effects of O2 on proteins and DNA. These precise conditions are essential for Dr. Janbandhu’s work as they accurately replicate the in vivo environment.

 

His work also see benefits when comparing the use of a Hypoxystation as opposed to using a CO2 incubator. Typically, cell culture work involves methods which include isolating cells under their usual physiologically relevant conditions and then working with them in “bench-top conditions” where cells are exposed to non-physiological oxygen. This can then lead to altered hypoxic response, metabolism, reactive oxygen species and DNA damage response. This metabolic stress introduces unknown outcomes and may lead to results inconsistent with physiological processes. Therefore, the precise control of oxygen levels in cell culture has been shown to be vital for reproducible and physiologically relevant results, transforming the working environment in Vaibhao’s lab.

 

As well as the precise controlling of conditions, Vaibhao likes the remote access feature on his H35 Hypoxystation. The remote access allows Vaibhao to log into his Hypoxystation’s touchscreen control whilst away from the unit, offering increased flexibility in his working methods. Additionally, he likes how he can view operating conditions, set parameters and change access levels remotely.

 

Dr. Janbandhu opted to purchase a Hypoxystation from Don Whitley Scientific for the level of service and specification we were able to provide in Australia. Other companies either couldn’t fulfil configuration requirements and didn’t provide sales and or service in Australia. Don Whitley Scientific’s office in Australia, provides both fantastic sales and service nationwide. Vaibhao also states that from “discussion with other research groups across the world we felt confident to go for a DWS Hypoxystation”.

 

Dr. Vaibhao Janbandhu has this to say about Don Whitley Scientific Pty Ltd: “I would like to thank your company personnel at the customer services division in Australia for their excellent support. Your Sales & Service Manager in Australia, Grant Shallcross, took care of all my queries in a jiffy!”. Vaibhao added that the funding for the purchase came from the James N Kirby Foundation and the Victor Chang Cardiac Research Institute.

 

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Neues System zur automatischen Auswertung von ID- und AST-Platten

ChromaZona

Die Firma Synoptics hat mit dem “ChromaZona” ein Produkt zur automatischen Auswertung von ID- und AST-Platten auf den Markt gebracht. Damit können verschiedene mikrobielle Arten auf chromogenen Petrischalen innerhalb von Sekunden identifiziert werden. Das Produkt wurde erstmals auf der Analytica in München der Öffentlichkeit präsentiert.

 

Das Gerät verfügt über zwei verschiedene Softwareprogramme,  die Chromogen ID und die eAST-Software. Die Chromogen ID ist eine einzigartige Farberkennungs-Software, die viele Mikroorganismen auf Medienträgern durch einen Klick automatisch erkennt. Mit der eAST Software (Easy Antibiotic Susceptibility Testing) werden bei der Analyse der Hemmhöfe und MIC-Streifen (inkl. Thermo Fisher) schnell die benötigten Antiobiotika zur Bekämpfung der Infektion ermittelt.

 

Das Gerät erkennt automatisch verschiedene Petrischalen mit einem Durchmesser von bis zu 150 mm. Optional kann der “ChromaZona” mit einem LIMS-System verbunden werden und weiterhin können die Berichte in Excel erstellt und gespeichert werden. Das System eigenet sich zur Nutzung in einer CFR Part 11 Umgebung. Zudem sind die jährlichen Softwareupdates ohne jährliche Lizenzgebühr frei verfügbar.