New Video – An Introduction to Don Whitley Scientific

New Video Don Whitley Scientific

Don Whitley Scientific is delighted to present a new corporate video showing some of the people and processes that have allowed the organisation to remain successful after 40 years in business.

 

This short video, produced by local company Rejuvenate Productions, gives an insight into how Don Whitley Scientific designs, manufactures and services equipment for laboratories throughout the world. Don Whitley Scientific is very proud of its “one-stop-shop” concept, something that is very much appreciated by customers.

 

The video starts in the research and development office where members of staff are designing new products and developing ideas for existing products. From there an insight is given into our CNC machine shop where key components are manufactured. The video progresses through the key stages of production, sales, installation and servicing.

 

We hope we have been able to provide a flavour of how we work with our customers to bring new and innovative solutions to the scientific community.

 

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Examining the Role of Autophagy in Hypoxic Tumours

Examining the Role of Autophagy

Hypoxystation users Tan et al. at the University of Toronto published a paper in June examining the significance of autophagy in cancer development (“Role of Autophagy as a Survival Mechanism for Hypoxic Cells in Tumors“, Neoplasia (2016) 18, 347-355). Autophagy as a means of recycling cell components is induced under stress conditions such as hypoxia, and Tan et al. investigated the correlation of hypoxia and autophagy in solid tumours in the context of resistance to cancer therapeutics.

 

Cells were cultured in the H35 Hypoxystation for up to 48 hours at hypoxia (0.2 %) and compared to cells grown at ambient oxygen level. Gene silencing of autophagy proteins ATG7 and BECLIN1 with shRNA resulted in decreased cell survival under hypoxia, and inhibition of autophagy with pantoprazole exacerbated the loss of viability in the knock-down cells under hypoxia, demonstrating the cyto-protective effects of these autophagy proteins. Using the Seahorse XFe Analyzer to assess oxygen consumption in wild-type and silenced cells, Dr. Tan’s lab found reduced respiration when autophagy is disrupted, possibly due to accumulation of dysfunctional mitochondria in these mutant cells. The H35 Hypoxystation  Dr. Tan’s lab used for these studies creates a closed environment with controlled temperature, humidity, CO2 and oxygen, in which cells are cultured and manipulated over the course of days and weeks without the need to transfer into ambient conditions. The combination of an Hypoxystation and an i2 Instrument Workstation is designed to accommodate the specific requirements of the Seahorse XFe Analyzer for the duration of the metabolism assays investigating oxygen consumption and extracellular acidification.

 

Please visit the Meintrup DWS Laborgeräte website for more published papers featuring the Whitley Workstation range or contact us to discuss your hypoxia needs.

New Option – Catalyst Monitoring System

Catalyst Monitoring System 1

This new facility is a very efficient and effective way of alerting users to both the condition of the catalyst and the anaerobic atmosphere in a Whitley Workstation. This patented application is an ingenious software based system, which requires only a minimal amount of unobtrusive hardware.

 

The Catalyst Monitoring System is a factory-fitted option for Whitley Anaerobic Workstations. It is set to conduct the test overnight so that when you arrive at the lab in the morning, a pop-up box on the touchscreen informs you of the status of the catalyst. ‘Green’ means all is fine and there is plenty of life left in the catalyst; ‘amber’ means the catalyst performance is reduced; and ‘red’ means that the catalyst should be changed to maintain good anaerobic conditions.

 

The system also allows for the test to be run manually if required. This test is very valuable because it confirms that the catalyst is working and that hydrogen is present in the atmosphere, both of which are essential to maintain strict anaerobic conditions.

 

This option can be used either on its own or in conjunction with the Whitley Anaerobic Conditions Monitor, which provides continuous monitoring of the level of oxygen present in your workstation.

 

 

Catalyst Monitoring System 2

Anwenderbericht über die Messung des aw-Wertes von Süßwaren

LabMaster

Die Firma DataSweet Online GmbH aus Solingen, ein Serviceanbieter für die weltweite Süßwarenindustrie hat im Rahmen eines mehrmonatigen Projektes den LabMaster-aw, ein Gerät zur Messung des aw-Wertes, getestet. Im Rahmen dieses Projektes wurden Süßwaren und Anwendungen wie z. B. Hartkaramelle, Gummi- und Gelee-Artikel oder Dragees von verschiedenen Herstellern getestet. Der LabMaster-aw wurde dabei als Überprüfungs- und Kontrollgerät getestet.

 

Das Ergebnis dieses Projektes wurde uns von Frau Pernot-Barry als Anwenderbericht zur Verfügung gestellt. Um den kompletten Bericht sehen zu können, klicken Sie HIER!

Whitley Workstation used in Clostridium difficile study

A35 Clostridium difficile study

The Whitley A35 Workstation has recently been used by a group at Monash University in a study relating to toxins affected by Clostridium difficile.

 

This very interesting research paper, entitled “CdtR Regulates TcdA and TcdB Production in Clostridium difficile“, was written by researchers at Monash University in Melbourne, Australia. The results from the study “establish CdtR as an important virulence regulator in two clinically important, epidemic strains of C. difficile, and further highlights the need to investigate regulatory mechanisms of important virulence factors in diverse strain backgrounds.”  The paper proves for the first time that “TcdA and TcdB production is linked to the production of CDT by a common regulatory mechanism”.

 

Click here to read the full paper


Whitley H35 Hypoxystation used in tumour study

KONICA MINOLTA DIGITAL CAMERA

Researchers from Oxford and Stanford universities have been using an Whitley H35 Hypoxystation to look at how the hypoxic conditions of tumours repress DNA repair pathways that protect against genomic instability. Hypoxystation users Leszczynska et al describe the interaction of DNA damage kinase ATM with the ATM interactor ATMIN within hypoxia as well as the downstream consequences for DNA repair. An abstract from the article is posted below, as well as a link to the full article.

 

ATM activation is induced at severe hypoxia as a result of replication stress, and was thought to be dependent on the ATM interactor ATMIN, especially in the absence of DNA damage. Using ATMIN siRNA, ATM inhibitors, and knock-out cell lines, the authors were able to establish that ATMIN is not required for the activation of ATM in response to hypoxia-induced replication stress, and that ATMIN is repressed at hypoxia, an effect mediated by both p53 and HIF-1. The cells were exposed to varying degrees of hypoxia, from mild (2%) down to extreme (0.1%) in an Whitley H35 Hypoxystation by Don Whitley Scientific. The closed cell culture environment created in the Hypoxystation mimics physiological conditions with regard to oxygen, CO2, temperature, and humidity and enables cancer researchers to obtain a clearer picture of in vivo processes. qPCR analysis of cells in response to hypoxia and exposure to inhibitors of proteasomal degradation indicate that the repressive effect of hypoxia is due to inhibition of translation as opposed to transcription or altered stability of ATMIN.

 

Using ATMIN siRNA at various levels of hypoxia, the authors found that loss of ATMIN impairs base excision repair BER and increases sensitivity to DMA damaging agents such as methyl methanesulfonate MMS. Decreased ATMIN levels also decrease the expression of dynein light chain LC8-type 1 DYNLL1, again in a p53-dependent manner. Thus, the authors have found a new link between tumor hypoxia and ATMIN-regulated DYNLL1 expression. Loss of DYNLL1 in hypoxic tumors affects ciliogenesis, mitosis, cellular localization of proteins, to name a few, and justifies further research into the roles of ATMIN and DYNLL1 in cancer.

 

The full paper can be read here – Mechanisms and consequences of ATMIN repression in hypoxic conditions: roles for p53 and HIF-1