HEPA-Filtration von Don Whitley Scientific

hepa-filter-300x169

Seit über 40 Jahren nutzt Don Whitley Scientific Erfahrung und Know-how, um sicherzustellen, dass die Produkte die Arbeitsmethoden für unsere Laborkunden einfacher machen. Da immer mehr Wert auf saubere Luft gelegt wird, kann die HEPA-Filtration eine zusätzliche Maßnahme sein, um sicherzustellen, dass die in den Whitley Arbeitsstationen durchgeführten Untersuchungen in einer Umgebung durchgeführt werden, die Partikelkonzentrationen über den in ISO 14644-1 Teil 1, Tabelle 1, angegebenen Werten erreicht. (Klasse 3) Klassifizierung der Luftreinheit.

 

Don Whitley Scientific hat Tests durchgeführt, die zeigen, welchen Unterschied ein HEPA-Filtersystem zur Atmosphäre einer Whitley Workstation macht. Don Whitley Scientific stellt seit über 40 Jahren Produkte her und die Einführung der HEPA-Filtration war ein logischer Schritt, um sicherzustellen, dass unsere Kunden eine Option haben, die den neuesten Labortrends und -anforderungen entspricht.

 

Die Vorteile des Whitley HEPA Filtersystems sind:

  • Das Whitley-System verwendet einen internen HEPA-Filter. Die Atmosphäre wird schnell und kontinuierlich gereinigt, da das gesamte interne atmosphärische Volumen jeder Whitley Workstation den Filter hunderte Male pro Stunde durchläuft.
  • Die Atmosphäre, die durch den Filter strömt, erzeugt einen laminaren, strömungsartigen Effekt, der etwa 94% der Breite der Kammer abdeckt.
  • Der Standort des Whitley HEPA-Filters innerhalb der Arbeitsstation stellt sicher, dass es nicht nass werden kann. Daher kann es nicht mit Feuchtigkeit gesättigt und unwirksam gemacht werden.

Don Whitley Scientific hat in Zusammenarbeit mit einem der weltweit führenden Filterhersteller einen Filter entwickelt, der speziell auf die Anforderungen anaerober Arbeitsstationen zugeschnitten ist. Durch Hinzufügen der HEPA-Filtertechnologie zur Arbeitsstation wird eine Umgebung geschaffen, in der mikrobiologische Proben und Zellkulturen frei von Kontaminationsrisiken wachsen können.

 

HEPA-Filtration wurde als Option nach jahrzehntelanger Erfahrung in der Mikrobiologie eingeführt. Wir sind zuversichtlich, dass unsere gründliche Herangehensweise an die Produktentwicklung und Produktprüfung zu einem HEPA-Filtersystem geführt hat, das allen anderen Systemen überlegen ist, die an Arbeitsplätzen mit positivem Druck und modifizierter Atmosphäre installiert sind. Das Video unten erklärt die Whitley HEPA-Filtration in weiteren Einzelheiten.

 

 

 

Entdecken Sie die Whitley HEPA Workstation Reihe.

 

Don Whitley Scientific wird nach Bingley ziehen

DWS Bingley

 

Nach 40 Jahren Produktion in Shipley hat Don Whitley Scientific Limited angekündigt, dass das Unternehmen in neue Räumlichkeiten in Bingley ziehen wird.

 

 

 

 

 

 

 

Geschäftsführer Paul Walton erklärt: Paul-Walton-150x150
“Unser Geschäft ist vor allem in den letzten zehn Jahren erheblich gewachsen, und obwohl wir die Otley Road Nr. 16 und 18 gekauft haben, sind wir unseren derzeitigen Anlagen immer noch entwachsen. Als Victoria Works zum Verkauf kam und 48.100 m² zur Verfügung stellte, wussten wir, dass dies der richtige Schritt für uns wäre. Das Bingley-Gelände wird uns den Raum bieten, um uns effizienter zu organisieren und noch Raum für Expansion zu haben. ”

 

 

 

 

 

Das neue Gebäude, direkt an der Bradford Road, wird von Eclipse Interiors umfassend renoviert, um es den Bedürfnissen des Unternehmens anzupassen. Es wird wesentlich mehr Lagerkapazitäten geben sowie neue Büros, eine maßgeschneiderte Produktionsfläche, ein Servicezentrum, ein F & E-Zwischengeschoss und verbesserte Personaleinrichtungen einschließlich einer großen, voll ausgestatteten, klimatisierten Kantine. Außerdem wird es einen Showroom für neue Produkte geben, in dem Kunden Demonstrationen der neuesten anaeroben und hypoxischen Workstation-Technologie sehen können.

 

Das Büro für Verkauf, Service und Marketing
Lagerbereich

 

 

Produkt Showroom

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Der Umzug findet vom 13. bis 17. Juni statt, so dass das Geschäft am Montag, den 18. Juni, von Bingley aus wieder in Betrieb genommen werden kann.

Cancer Cell Study Carried Out At The University Of Bradford

cancer-cell-study-uni-bradford

A paper entitled “Plysialic acid sustains cancer cell survival and migratory capacity in a hypoxic environment” has been published by researchers at The University of Bradford and The University of Huddersfield. The study looks at Polysialic acid (a unique carbohydrate polymer expressed on the surface of neuronal cell adhesion molecules) and its association with tumour cell and adhesion in hypoxia. Their findings provide the first evidence that polySia expression sustains migratory capacity and is associated with tumour cell survival in hypoxia.

 

A key part of the study involved the use of a Whitley H35 Hypoxystation. The importance of using a piece of equipment such as a Whitley Hypoxystation is down to hypoxia having a profound effect on cancer cell growth as it occurs in poorly vascularised areas of tumours. Klaus Pors, Senior Lecturer In Chemical Biology, provided the below quote.

 

Dr Robert Falconer and colleagues are studying polysialyl transferases (polySTs), responsible for the biosynthesis of polysialic acid (polySia), as a potential antimetastatic therapeutic strategy. PolySia is a unique carbohydrate polymer capable of modulating cell-cell and cell-matrix adhesion, migration, invasion and metastasis in a number of cancers. In this study we have employed Don Whitley H35 Hypoxystation to analyse how polySia sustains cancer cell survival and migratory capacity in a hypoxic environment. We believe these results contribute significantly to our understanding of how polySia supports an aggressive phenotype and further studies are underway to underpin these findings in a therapeutic context.

 

Furthermore, the paper also states that the “results have significant potential implications for polyST inhibition as an anti-metastatic therapeutic strategy and for targeting hypoxic cancer cells”.

 

The group are set to continue this line of work, using the Whitley H35 Hypoxystation, keep an eye on the Meintrup DWS blog for more articles on this.

Physiological Oxygen is Healthier for Cell Cultures

Physiological Oxygen is Healthier for Cell Cultures

Drs. Timpano and Uniacke, Hypoxystation users at University of Guelph in Ontario, have published a very thorough study examining the molecular basis of cells’ reactions to differing levels of hypoxia. In their paper “Human Cells Cultured Under Physiological Oxygen Utilize Two Cap-binding Proteins to Recruit Distinct mRNAs for Translation” (Journal of Biological Chemistry 291:20; 2016), they examine 2 different translation initiation proteins, eiF4E and eiF4E2, that are activated under either high (>8% O2) or low (<1% O2) oxygen levels, with the aid of mTORC1 or HIF-2α, respectively, and activated simultaneously in an area of low- to mid-level physioxia (1-8% O2). Timpano and Uniacke were able to stably and accurately create low oxygen in their Hypoxystation by Hypoxygen, which provides a closed workstation environment that enables researchers to culture and manipulate cells inside the chamber through gloveless sleeves, eliminating the negative consequences of spikes of higher oxygen and lower temperatures encountered in an incubator as cell cultures are growing. Their research into translational modulation of the proteome using the Hypoxystation gives seminal insights into physioxia as the natural condition for cells, both in vitro and in vivo.

“Culturing cells in ambient air could be far from physiological with respect to oxygen. Oxygen is a surprisingly neglected factor (in cell culture)” – Dr Timpano and Dr Uniacke, University of Guelph, Ontario, Canada

Through polysome association experiments with cells growing at ambient air versus lower oxygen levels of 1%, 3%, 5%, and 8%, RNA analysis, and m7-GTP cap-binding assays, Timpano and Uniacke were able to demonstrate that the oxygen concentration in the workstation was sufficient to either repress or increase the activity of eiF4E and eiF4E2, reflecting mechanisms that occur during development but also during tumor progression and in ischemic diseases. Cells can reversibly cycle between utilisation of the eiF4E protein, which preferentially binds to the 5′ TOP mRNA’s at >8% O2 and is impaired at hypoxia, and eiF4E2, which is active at <1% O2 and utilizes binding motifs in the 3′ UTR of the mRNA. The eIF4E type of mRNA’s code for housekeeping proteins while the eIF4E2-dependant mRNA’s encode signaling proteins needed to respond to environmental signals, allowing cells to control translation dynamically and giving cancer cells an edge during tumor progression, as hypoxia increases.

 

Read more

Examining the Role of Autophagy in Hypoxic Tumours

Examining the Role of Autophagy

Hypoxystation users Tan et al. at the University of Toronto published a paper in June examining the significance of autophagy in cancer development (“Role of Autophagy as a Survival Mechanism for Hypoxic Cells in Tumors“, Neoplasia (2016) 18, 347-355). Autophagy as a means of recycling cell components is induced under stress conditions such as hypoxia, and Tan et al. investigated the correlation of hypoxia and autophagy in solid tumours in the context of resistance to cancer therapeutics.

 

Cells were cultured in the H35 Hypoxystation for up to 48 hours at hypoxia (0.2 %) and compared to cells grown at ambient oxygen level. Gene silencing of autophagy proteins ATG7 and BECLIN1 with shRNA resulted in decreased cell survival under hypoxia, and inhibition of autophagy with pantoprazole exacerbated the loss of viability in the knock-down cells under hypoxia, demonstrating the cyto-protective effects of these autophagy proteins. Using the Seahorse XFe Analyzer to assess oxygen consumption in wild-type and silenced cells, Dr. Tan’s lab found reduced respiration when autophagy is disrupted, possibly due to accumulation of dysfunctional mitochondria in these mutant cells. The H35 Hypoxystation  Dr. Tan’s lab used for these studies creates a closed environment with controlled temperature, humidity, CO2 and oxygen, in which cells are cultured and manipulated over the course of days and weeks without the need to transfer into ambient conditions. The combination of an Hypoxystation and an i2 Instrument Workstation is designed to accommodate the specific requirements of the Seahorse XFe Analyzer for the duration of the metabolism assays investigating oxygen consumption and extracellular acidification.

 

Please visit the Meintrup DWS Laborgeräte website for more published papers featuring the Whitley Workstation range or contact us to discuss your hypoxia needs.

Whitley Workstation used in Clostridium difficile study

A35 Clostridium difficile study

The Whitley A35 Workstation has recently been used by a group at Monash University in a study relating to toxins affected by Clostridium difficile.

 

This very interesting research paper, entitled “CdtR Regulates TcdA and TcdB Production in Clostridium difficile“, was written by researchers at Monash University in Melbourne, Australia. The results from the study “establish CdtR as an important virulence regulator in two clinically important, epidemic strains of C. difficile, and further highlights the need to investigate regulatory mechanisms of important virulence factors in diverse strain backgrounds.”  The paper proves for the first time that “TcdA and TcdB production is linked to the production of CDT by a common regulatory mechanism”.

 

Click here to read the full paper


Paper published involving use of Whitley Anaerobic Workstation

Fallstudie Arbeitsstationen und Spiralplater

A paper has been published describing the effects of oxygen limitation on food-borne pathogen Listeria monocytogenes. The study was conducted by Carol Philips’ group at The University of Northampton and involved the use of a Whitley Anaerobic Workstation as well as a Whitley Automated Spiral Plater. The workstation was used to incubate the samples which were then plated onto BHI (Brain Heart Infusion) agar using the spiral plater.

 

This is just one of the areas of microbiology where Don Whitley Scientific equipment can be beneficial. Due to the level of atmospheric control on offer, Whitley Workstations are used across the broad spectrum of microbiology and can also be used in other scientific disciplines.

 

The Whitley Workstation range includes various sizes of workstations, accommodating 250 to 1200 petri dishes. Other products on offer from Don Whitley Scientific can be used in conjunction with our workstations, for example in this study a Whitley Automated Spiral Plater was also used.

 

Click here to read the full study

Anwenderbericht für den LabTouch aW

Microsoft Word - OttoBeier_LabTouch_Fruchtschnitten

Optimizing oxygen conditions enhances stem cell recovery

Two very recent papers, published in Cell and Regenerative Medicine, have described research into the advantages of reducing the oxygen exposure stem cells experience during isolation and cultivation, and the cellular mechanisms involved in translating hypoxia into increased stem cell survival and enhanced transplantation efficacy. The data presented by Mantel et al. on hematopoietic stem cells, and by Kay et al. on mesenchymal stem cells, caused quite a stir at the recent ISSCR meeting in Stockholm, as attendees discussed the implications for their own work.

Alasdair Kay and co-workers at Keele University evaluated the impact of various oxygen, glucose and serum levels on culturing human bone marrow-derived MSC’s. They found that the application of more physiological conditions, e.g. 2% oxygen instead of 21% O2 ambient, resulted in significantly increased cell yields; the highest yields were achieved in the stringently controlled hypoxic environment of a workstation. The authors attribute this to deoxygenation/reoxygenation cycles and very brief air exposure for cells cultured in an incubator, as compared to the controlled atmosphere in the workstation. Transcriptome analysis of hMSC’s cultured at 2% (incubator vs workstation) and 21% oxygen revealed scores of genes that were up or downregulated with very little overlap among the subsets. Kay et al. have identified downregulation of bone morphogenetic protein BMP2 expression, modulation of chemokine (C-X-C motif) receptor CXCR2 signaling, and LEPR upregulation as causative factors for enhanced hMSC isolation at hypoxia.

The term extraphysiologic oxygen shock/stress EPHOSS for hematopoietic stem cells experiencing “hyperoxia” outside of their natural niches is introduced by Mantel et al. from Indiana University. They delineated the detrimental effects of even brief exposure of bone marrow and cord blood to ambient conditions as compared to harvesting and processing HSC’s at constant 3% hypoxia. Up to 5-fold increased cell numbers and improved engraftment of transplanted HSC’s as a result of hypoxic conditions led the authors to question the relevance of the work currently being carried out at ambient oxygen. They attribute the beneficial effects of low oxygen on the yield and function of BM and CB stem cells to reduced production of reactive oxygen species ROS, implicating the mitochondrial permeability transition pore MPTP, cyclophilin CypD, and p53 in protection from EPHOSS. Mantel et al. predict that hypoxic processing will be significant for many other cell types and phenomena such as metabolism of cancer cells and stem cell aging.

This article was written by Burga Kalz Fuller, Product Manager at HypOxygen.

 

Alun Kitsell, Burga Kalz Fuller and Daniel Secker
Alun Kitsell, Burga Kalz Fuller and Daniel Secker with a Whitley HI35 Hypoxystation

 

  1. Kay et al. “BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation” 2015 Med. 10(2), 109-125
  2. Mantel et al. “Enhancing hematopoietic stem cell transplantation efficacy by mitigating oxygen shock” 2015 Cell 161, 1553-1565

 

Queens University Belfast

A research at QUB using the Whitley A45 Workstation

Groundbreaking research conducted at Queen’s University Belfast’s School of Pharmacy and the Centre for Infection and Immunity has led to new knowledge about cystic fibrosis (CF) that is being applied to patient care.

This video, produced by Video Services at QUB, explains new clinical trials where CF patients are participating in a clinical study aimed at bringing medicines and treatments to people faster. An important recent finding was that the lungs of people with CF are host to many different types of bacteria. The team are working to identify which ones may cause problems for people with CF. As part of this identification project, patient samples are processed in the laboratory in anaerobic workstations (the video shows staff using Whitley A35 and A45 Workstations).

The university is also working on better ways of delivering drugs to CF lungs, including the use of nanoparticles.