Don Whitley Scientific wird nach Bingley ziehen

DWS Bingley

 

Nach 40 Jahren Produktion in Shipley hat Don Whitley Scientific Limited angekündigt, dass das Unternehmen in neue Räumlichkeiten in Bingley ziehen wird.

 

 

 

 

 

 

 

Geschäftsführer Paul Walton erklärt: Paul-Walton-150x150
“Unser Geschäft ist vor allem in den letzten zehn Jahren erheblich gewachsen, und obwohl wir die Otley Road Nr. 16 und 18 gekauft haben, sind wir unseren derzeitigen Anlagen immer noch entwachsen. Als Victoria Works zum Verkauf kam und 48.100 m² zur Verfügung stellte, wussten wir, dass dies der richtige Schritt für uns wäre. Das Bingley-Gelände wird uns den Raum bieten, um uns effizienter zu organisieren und noch Raum für Expansion zu haben. ”

 

 

 

 

 

Das neue Gebäude, direkt an der Bradford Road, wird von Eclipse Interiors umfassend renoviert, um es den Bedürfnissen des Unternehmens anzupassen. Es wird wesentlich mehr Lagerkapazitäten geben sowie neue Büros, eine maßgeschneiderte Produktionsfläche, ein Servicezentrum, ein F & E-Zwischengeschoss und verbesserte Personaleinrichtungen einschließlich einer großen, voll ausgestatteten, klimatisierten Kantine. Außerdem wird es einen Showroom für neue Produkte geben, in dem Kunden Demonstrationen der neuesten anaeroben und hypoxischen Workstation-Technologie sehen können.

 

Das Büro für Verkauf, Service und Marketing
Lagerbereich

 

 

Produkt Showroom

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Der Umzug findet vom 13. bis 17. Juni statt, so dass das Geschäft am Montag, den 18. Juni, von Bingley aus wieder in Betrieb genommen werden kann.

Meintrup DWS bei der Analytica

analytica-logo

 

Vom 10. – 13. April waren wir mit unserer englischen Muttergesellschaft Don Whitley Scientific in München auf der 26. Analytica vertreten, um dort unsere Produkte auszustellen. Die Analytica ist eine internationale Fachmesse für Labortechnik, die alle Aspekte moderner Analytik in Industrie und Forschung vereint.

 

Meintrup DWS und Don Whitley Scientific zeigten auf dem Messestand eine breite Palette von Produkten. Dazu gehörten unter anderem der Spiralplater Whitley WASP Touch und die anaerobe Arbeitsstation Whitley A55. Ebenfalls ausgestellt waren verschiedene Geräte zur Probenvorbereitung, wie zum Beispiel der Luftkeimsammler Airwel und der Nährmedienpräparator Mediawel und diverse aW-Messgeräte.

 

Auch in diesem Jahr war die Teilnahme bei der Analytica wieder ein voller Erfolg und wir freuen uns aufs nächste Mal!

 

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Cancer Cell Study Carried Out At The University Of Bradford

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A paper entitled “Plysialic acid sustains cancer cell survival and migratory capacity in a hypoxic environment” has been published by researchers at The University of Bradford and The University of Huddersfield. The study looks at Polysialic acid (a unique carbohydrate polymer expressed on the surface of neuronal cell adhesion molecules) and its association with tumour cell and adhesion in hypoxia. Their findings provide the first evidence that polySia expression sustains migratory capacity and is associated with tumour cell survival in hypoxia.

 

A key part of the study involved the use of a Whitley H35 Hypoxystation. The importance of using a piece of equipment such as a Whitley Hypoxystation is down to hypoxia having a profound effect on cancer cell growth as it occurs in poorly vascularised areas of tumours. Klaus Pors, Senior Lecturer In Chemical Biology, provided the below quote.

 

Dr Robert Falconer and colleagues are studying polysialyl transferases (polySTs), responsible for the biosynthesis of polysialic acid (polySia), as a potential antimetastatic therapeutic strategy. PolySia is a unique carbohydrate polymer capable of modulating cell-cell and cell-matrix adhesion, migration, invasion and metastasis in a number of cancers. In this study we have employed Don Whitley H35 Hypoxystation to analyse how polySia sustains cancer cell survival and migratory capacity in a hypoxic environment. We believe these results contribute significantly to our understanding of how polySia supports an aggressive phenotype and further studies are underway to underpin these findings in a therapeutic context.

 

Furthermore, the paper also states that the “results have significant potential implications for polyST inhibition as an anti-metastatic therapeutic strategy and for targeting hypoxic cancer cells”.

 

The group are set to continue this line of work, using the Whitley H35 Hypoxystation, keep an eye on the Meintrup DWS blog for more articles on this.

Physiological Oxygen is Healthier for Cell Cultures

Physiological Oxygen is Healthier for Cell Cultures

Drs. Timpano and Uniacke, Hypoxystation users at University of Guelph in Ontario, have published a very thorough study examining the molecular basis of cells’ reactions to differing levels of hypoxia. In their paper “Human Cells Cultured Under Physiological Oxygen Utilize Two Cap-binding Proteins to Recruit Distinct mRNAs for Translation” (Journal of Biological Chemistry 291:20; 2016), they examine 2 different translation initiation proteins, eiF4E and eiF4E2, that are activated under either high (>8% O2) or low (<1% O2) oxygen levels, with the aid of mTORC1 or HIF-2α, respectively, and activated simultaneously in an area of low- to mid-level physioxia (1-8% O2). Timpano and Uniacke were able to stably and accurately create low oxygen in their Hypoxystation by Hypoxygen, which provides a closed workstation environment that enables researchers to culture and manipulate cells inside the chamber through gloveless sleeves, eliminating the negative consequences of spikes of higher oxygen and lower temperatures encountered in an incubator as cell cultures are growing. Their research into translational modulation of the proteome using the Hypoxystation gives seminal insights into physioxia as the natural condition for cells, both in vitro and in vivo.

“Culturing cells in ambient air could be far from physiological with respect to oxygen. Oxygen is a surprisingly neglected factor (in cell culture)” – Dr Timpano and Dr Uniacke, University of Guelph, Ontario, Canada

Through polysome association experiments with cells growing at ambient air versus lower oxygen levels of 1%, 3%, 5%, and 8%, RNA analysis, and m7-GTP cap-binding assays, Timpano and Uniacke were able to demonstrate that the oxygen concentration in the workstation was sufficient to either repress or increase the activity of eiF4E and eiF4E2, reflecting mechanisms that occur during development but also during tumor progression and in ischemic diseases. Cells can reversibly cycle between utilisation of the eiF4E protein, which preferentially binds to the 5′ TOP mRNA’s at >8% O2 and is impaired at hypoxia, and eiF4E2, which is active at <1% O2 and utilizes binding motifs in the 3′ UTR of the mRNA. The eIF4E type of mRNA’s code for housekeeping proteins while the eIF4E2-dependant mRNA’s encode signaling proteins needed to respond to environmental signals, allowing cells to control translation dynamically and giving cancer cells an edge during tumor progression, as hypoxia increases.

 

Read more

Examining the Role of Autophagy in Hypoxic Tumours

Examining the Role of Autophagy

Hypoxystation users Tan et al. at the University of Toronto published a paper in June examining the significance of autophagy in cancer development (“Role of Autophagy as a Survival Mechanism for Hypoxic Cells in Tumors“, Neoplasia (2016) 18, 347-355). Autophagy as a means of recycling cell components is induced under stress conditions such as hypoxia, and Tan et al. investigated the correlation of hypoxia and autophagy in solid tumours in the context of resistance to cancer therapeutics.

 

Cells were cultured in the H35 Hypoxystation for up to 48 hours at hypoxia (0.2 %) and compared to cells grown at ambient oxygen level. Gene silencing of autophagy proteins ATG7 and BECLIN1 with shRNA resulted in decreased cell survival under hypoxia, and inhibition of autophagy with pantoprazole exacerbated the loss of viability in the knock-down cells under hypoxia, demonstrating the cyto-protective effects of these autophagy proteins. Using the Seahorse XFe Analyzer to assess oxygen consumption in wild-type and silenced cells, Dr. Tan’s lab found reduced respiration when autophagy is disrupted, possibly due to accumulation of dysfunctional mitochondria in these mutant cells. The H35 Hypoxystation  Dr. Tan’s lab used for these studies creates a closed environment with controlled temperature, humidity, CO2 and oxygen, in which cells are cultured and manipulated over the course of days and weeks without the need to transfer into ambient conditions. The combination of an Hypoxystation and an i2 Instrument Workstation is designed to accommodate the specific requirements of the Seahorse XFe Analyzer for the duration of the metabolism assays investigating oxygen consumption and extracellular acidification.

 

Please visit the Meintrup DWS Laborgeräte website for more published papers featuring the Whitley Workstation range or contact us to discuss your hypoxia needs.

Whitley H35 Hypoxystation used in tumour study

KONICA MINOLTA DIGITAL CAMERA

Researchers from Oxford and Stanford universities have been using an Whitley H35 Hypoxystation to look at how the hypoxic conditions of tumours repress DNA repair pathways that protect against genomic instability. Hypoxystation users Leszczynska et al describe the interaction of DNA damage kinase ATM with the ATM interactor ATMIN within hypoxia as well as the downstream consequences for DNA repair. An abstract from the article is posted below, as well as a link to the full article.

 

ATM activation is induced at severe hypoxia as a result of replication stress, and was thought to be dependent on the ATM interactor ATMIN, especially in the absence of DNA damage. Using ATMIN siRNA, ATM inhibitors, and knock-out cell lines, the authors were able to establish that ATMIN is not required for the activation of ATM in response to hypoxia-induced replication stress, and that ATMIN is repressed at hypoxia, an effect mediated by both p53 and HIF-1. The cells were exposed to varying degrees of hypoxia, from mild (2%) down to extreme (0.1%) in an Whitley H35 Hypoxystation by Don Whitley Scientific. The closed cell culture environment created in the Hypoxystation mimics physiological conditions with regard to oxygen, CO2, temperature, and humidity and enables cancer researchers to obtain a clearer picture of in vivo processes. qPCR analysis of cells in response to hypoxia and exposure to inhibitors of proteasomal degradation indicate that the repressive effect of hypoxia is due to inhibition of translation as opposed to transcription or altered stability of ATMIN.

 

Using ATMIN siRNA at various levels of hypoxia, the authors found that loss of ATMIN impairs base excision repair BER and increases sensitivity to DMA damaging agents such as methyl methanesulfonate MMS. Decreased ATMIN levels also decrease the expression of dynein light chain LC8-type 1 DYNLL1, again in a p53-dependent manner. Thus, the authors have found a new link between tumor hypoxia and ATMIN-regulated DYNLL1 expression. Loss of DYNLL1 in hypoxic tumors affects ciliogenesis, mitosis, cellular localization of proteins, to name a few, and justifies further research into the roles of ATMIN and DYNLL1 in cancer.

 

The full paper can be read here – Mechanisms and consequences of ATMIN repression in hypoxic conditions: roles for p53 and HIF-1

Whitley H35 Hypoxystation used in heart regeneration project

H35 Australia 1

Dr. Vaibhao Janbandhu is a Postdoctoral Research Scientist at the Victor Chang Cardiac Research Institute (VCCR) in Sydney. He has been in contact with Don Whitley Scientific to explain how his lab’s work has benefited from the use of a Whitley H35 Hypoxystation. Vaibhao uses the Hypoxystation to isolate, culture and characterise adult cardiac stem cells (CSCs).

 

Dr. Janbandhu had already been using a H35 that was set up at the institute for almost three years before he got his own unit installed last year. Specifically, his project is to find new ways to stimulate heart regeneration during ageing and after heart attack. For this he needs a way to isolate, culture and characterise adult CSCs. In Vaibhao’s words the H35 Hypoxystation seems well suited for this application: “the DWS Hypoxystation provides a highly stabilised HEPA-filtered environment in which levels of oxygen, carbon dioxide, temperature and humidity are precisely controlled and it will be an integral part of the project to advance the project aims”.

 

Vaibhao explains that mammalian stem cells reside in a specialised cellular microenvironment. This niche and the stem cell niche is characterised by a low partial oxygen pressure. This hypoxic niche protects stem cells from deleterious effects of O2 on proteins and DNA. These precise conditions are essential for Dr. Janbandhu’s work as they accurately replicate the in vivo environment.

 

His work also see benefits when comparing the use of a Hypoxystation as opposed to using a CO2 incubator. Typically, cell culture work involves methods which include isolating cells under their usual physiologically relevant conditions and then working with them in “bench-top conditions” where cells are exposed to non-physiological oxygen. This can then lead to altered hypoxic response, metabolism, reactive oxygen species and DNA damage response. This metabolic stress introduces unknown outcomes and may lead to results inconsistent with physiological processes. Therefore, the precise control of oxygen levels in cell culture has been shown to be vital for reproducible and physiologically relevant results, transforming the working environment in Vaibhao’s lab.

 

As well as the precise controlling of conditions, Vaibhao likes the remote access feature on his H35 Hypoxystation. The remote access allows Vaibhao to log into his Hypoxystation’s touchscreen control whilst away from the unit, offering increased flexibility in his working methods. Additionally, he likes how he can view operating conditions, set parameters and change access levels remotely.

 

Dr. Janbandhu opted to purchase a Hypoxystation from Don Whitley Scientific for the level of service and specification we were able to provide in Australia. Other companies either couldn’t fulfil configuration requirements and didn’t provide sales and or service in Australia. Don Whitley Scientific’s office in Australia, provides both fantastic sales and service nationwide. Vaibhao also states that from “discussion with other research groups across the world we felt confident to go for a DWS Hypoxystation”.

 

Dr. Vaibhao Janbandhu has this to say about Don Whitley Scientific Pty Ltd: “I would like to thank your company personnel at the customer services division in Australia for their excellent support. Your Sales & Service Manager in Australia, Grant Shallcross, took care of all my queries in a jiffy!”. Vaibhao added that the funding for the purchase came from the James N Kirby Foundation and the Victor Chang Cardiac Research Institute.

 

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Hypoxische Mikroumgebungen – Cell Press Nukleus

DWS Hypoxygen

Don Whitley Scientific und HypOxygen, der amerikanische Distributor von Don Whitley, haben sich mit Cell Press zusammengeschlossen, um ein forschungsbasiertes Nukleus mit Fokus auf Zellen in Umgebung mit geringem Sauerstoff zu fördern. Das Cell Press Webinar für „Hypoxische Mikroumgebungen“ war eine großartige Möglichkeit, um die Vorträge von Randall Johnson vom Karolinska Institut über die hypoxischen Reaktionen im Zusammenhang mit Metastasen sowie von Hannele Ruohola-Baker von der Universität Washington über die Rolle von hypoxischen Faktoren in der Reprogrammierung von somatischen Zellen zu hören.

 

Wir glauben, unser Engagement, das zuverlässigste, präziseste und robusteste Instrument für physiologische Zellkulturen anzubieten, hilft unseren Anwendern ihre Forschungen durchzuführen, und wir sind froh ein kleiner Teil davon zu sein. Whitley Hypoxystationen schaffen eine in vivo Umgebung für die Zellkultivierung und Manipulation über die Dauer der Inkubation einschließlich funktionale Studien über geringen Sauerstoff.

 

Wenn Sie das Webinar noch einmal aufrufen möchten, können Sie über diesen Link darauf zugreifen. Das Cell Press Nukleus, das ausgewählte Dokumente zu den Themen Krebs, Entzündungen, Stammzellen und Nervenentwicklung einschließt, bietet wertvolle Erkenntnisse der Hypoxie und stellt zudem Verbindungen zu Funktion und Krankheit her.

CellPress

Biotechnica 2015

Biotechnica 2015

Auch in diesem Jahr waren wir wieder auf der Biotechnica, der Weltleitmesse für Biotechnologie und Life Sciences, vertreten. Die Messe fand vom 6. bis zum 8. Oktober in Hannover statt. Als große Neuerung wurde dieses Jahr die hypoxische i2 Arbeitsstation präsentiert. Diese Station wurde entwickelt um große Instrumente in hypoxischer Atmosphäre unterbringen zu können. Zudem kann sie als alleinstehende Einheit oder mit Hilfe eines Transfertunnels mit einer Whitley H35, H45 oder H135 Arbeitsstation kombiniert werden. Dies wurde auf der Messe durch eine Kombination mit der H35 dargestellt.

 

Eine weitere Sehenswürdigkeit war das Kolonienzählgerät „ProtoCOL“. Dieser kann dazu genutzt werden, Kolonien von Bakterien auf Petrischalen automatisch, also ohne aufwendiges manuelles Zählen, zu erfassen und bietet so eine enorme Zeitersparnis. Weiterhin wurden die aktuellsten Geräte zur Messung der Wasseraktivität in Lebensmitteln, der LabMaster, der LabTouch und der LabSwift, präsentiert.

 

Der Stand war auch dieses Jahr gut besucht und es wurden interessante Gespräche, sowohl mit Stammkunden als auch mit neuen Interessenten, geführt.