Don Whitley Scientific wird nach Bingley ziehen

DWS Bingley

 

Nach 40 Jahren Produktion in Shipley hat Don Whitley Scientific Limited angekündigt, dass das Unternehmen in neue Räumlichkeiten in Bingley ziehen wird.

 

 

 

 

 

 

 

Geschäftsführer Paul Walton erklärt: Paul-Walton-150x150
“Unser Geschäft ist vor allem in den letzten zehn Jahren erheblich gewachsen, und obwohl wir die Otley Road Nr. 16 und 18 gekauft haben, sind wir unseren derzeitigen Anlagen immer noch entwachsen. Als Victoria Works zum Verkauf kam und 48.100 m² zur Verfügung stellte, wussten wir, dass dies der richtige Schritt für uns wäre. Das Bingley-Gelände wird uns den Raum bieten, um uns effizienter zu organisieren und noch Raum für Expansion zu haben. ”

 

 

 

 

 

Das neue Gebäude, direkt an der Bradford Road, wird von Eclipse Interiors umfassend renoviert, um es den Bedürfnissen des Unternehmens anzupassen. Es wird wesentlich mehr Lagerkapazitäten geben sowie neue Büros, eine maßgeschneiderte Produktionsfläche, ein Servicezentrum, ein F & E-Zwischengeschoss und verbesserte Personaleinrichtungen einschließlich einer großen, voll ausgestatteten, klimatisierten Kantine. Außerdem wird es einen Showroom für neue Produkte geben, in dem Kunden Demonstrationen der neuesten anaeroben und hypoxischen Workstation-Technologie sehen können.

 

Das Büro für Verkauf, Service und Marketing
Lagerbereich

 

 

Produkt Showroom

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Der Umzug findet vom 13. bis 17. Juni statt, so dass das Geschäft am Montag, den 18. Juni, von Bingley aus wieder in Betrieb genommen werden kann.

Optimizing oxygen conditions enhances stem cell recovery

Two very recent papers, published in Cell and Regenerative Medicine, have described research into the advantages of reducing the oxygen exposure stem cells experience during isolation and cultivation, and the cellular mechanisms involved in translating hypoxia into increased stem cell survival and enhanced transplantation efficacy. The data presented by Mantel et al. on hematopoietic stem cells, and by Kay et al. on mesenchymal stem cells, caused quite a stir at the recent ISSCR meeting in Stockholm, as attendees discussed the implications for their own work.

Alasdair Kay and co-workers at Keele University evaluated the impact of various oxygen, glucose and serum levels on culturing human bone marrow-derived MSC’s. They found that the application of more physiological conditions, e.g. 2% oxygen instead of 21% O2 ambient, resulted in significantly increased cell yields; the highest yields were achieved in the stringently controlled hypoxic environment of a workstation. The authors attribute this to deoxygenation/reoxygenation cycles and very brief air exposure for cells cultured in an incubator, as compared to the controlled atmosphere in the workstation. Transcriptome analysis of hMSC’s cultured at 2% (incubator vs workstation) and 21% oxygen revealed scores of genes that were up or downregulated with very little overlap among the subsets. Kay et al. have identified downregulation of bone morphogenetic protein BMP2 expression, modulation of chemokine (C-X-C motif) receptor CXCR2 signaling, and LEPR upregulation as causative factors for enhanced hMSC isolation at hypoxia.

The term extraphysiologic oxygen shock/stress EPHOSS for hematopoietic stem cells experiencing “hyperoxia” outside of their natural niches is introduced by Mantel et al. from Indiana University. They delineated the detrimental effects of even brief exposure of bone marrow and cord blood to ambient conditions as compared to harvesting and processing HSC’s at constant 3% hypoxia. Up to 5-fold increased cell numbers and improved engraftment of transplanted HSC’s as a result of hypoxic conditions led the authors to question the relevance of the work currently being carried out at ambient oxygen. They attribute the beneficial effects of low oxygen on the yield and function of BM and CB stem cells to reduced production of reactive oxygen species ROS, implicating the mitochondrial permeability transition pore MPTP, cyclophilin CypD, and p53 in protection from EPHOSS. Mantel et al. predict that hypoxic processing will be significant for many other cell types and phenomena such as metabolism of cancer cells and stem cell aging.

This article was written by Burga Kalz Fuller, Product Manager at HypOxygen.

 

Alun Kitsell, Burga Kalz Fuller and Daniel Secker
Alun Kitsell, Burga Kalz Fuller and Daniel Secker with a Whitley HI35 Hypoxystation

 

  1. Kay et al. “BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation” 2015 Med. 10(2), 109-125
  2. Mantel et al. “Enhancing hematopoietic stem cell transplantation efficacy by mitigating oxygen shock” 2015 Cell 161, 1553-1565

 

Clinical Microbiology of Anaerobes – Course Success

Don Whitley Scientific recently sponsored a two day residential course delivered by the UK Anaerobe Reference Unit, Public Health Wales. The Practical & Clinical Microbiology of Anaerobes Course was held on 25-26 June in Cardiff. The event served as a refresher on culturing, identifying and being aware of the clinical importance of anaerobes. 

The 20 places available on the course were filled within a few weeks of the event being promoted, proving that the subject matter is of real interest to today’s microbiologists. Delegates came from all over the UK: from Inverness to Truro!

The aims of the course were:

  • To promote an understanding and awareness of anaerobic bacteria in clinical material
  • To gain an insight into the relevance of anaerobic bacteria in clinical microbiology
  • For delegates to achieve a basic level of competence in methods used for the isolation and identification of anaerobes
  • To improve the standards of anaerobic microbiology in clinical laboratories.

Trefor Morris, Lead Scientist of the UKARU, said:

“I am very proud of the commitment and dedication of my team in delivering such an excellent course. The positive feedback shows that we must continue to use our expertise to reinforce the importance of anaerobic bacteria, particularly in the face of the challenges of antimicrobial resistance. Indeed the fact that the course is already almost full for next year is testament to the level of interest and its unique nature.”

“I am also particularly happy that the delegates found the course friendly and interesting highlighted by the comments below:”

“A friendly, knowledgeable and very interesting course that I would recommend to anyone.”

“Very friendly atmosphere throughout the course, very knowledgeable and helpful staff during practical.”

“Really enjoyed the course, excellently organised and learnt lots to take back to my laboratory.”

“A fantastic course, the human clinical case presentations were very interesting.”

 

Don Whitley Scientific arranged for the use of a Whitley A55 Anaerobic Workstation for the course.

 

A Don Whitley A55 Workstation
A Whitley A55 Anaerobic Workstation

SfAM Summer Conference

Don Whitley Scientific exhibited at this year’s Society for Applied Microbiology (SfAM) Summer Conference, which was held in Dublin on 29 June to 2 July. The event was entitled “Fermented Foods and Beverages” and we took along a Protos 3 Automated Colony Counter and Whitley Automated Spiral Plater (WASP).

SfAM ran a competition for delegates and our donated prize, a hamper filled with goodies manufactured in Yorkshire, was won by Ogechukwu Elizabeth Okpalanozie of the University Of Lagos (pictured with Don Whitley).

73. Jahreskongress der SGM

73. Jahreskongress der Schweizerischen Gesellschaft für Mikrobiologie (SGM)

Am 28. und 29. Mai 2015 fanden Sie uns erstmals  im Palazzo dei Congressi in Lugano zu folgender Veranstaltung: 73. Jahreskongress der Schweizerischen Gesellschaft für Mikrobiologie (SGM). Bereits in den letzten Jahren hatten wir an den SGM-Kongressen teilgenommen, aber dieses Jahr war es für uns und besonders für Herrn Nordbruch das erste Mal in Lugano. Selbstverständlich hat er das schöne Wetter genauso wie die Umgebung genossen. Angereist ist er übrigens mit seinem neuen Firmenwagen. Mit diesem is es nun viel schneller möglich, Ihnen unsere Gerät näher zu bringen. Wir kommen zu Ihnen und können Ihnen nun endlich vor Ort unsere großen Arbeitsstationen vorstellen.

 

Hypoxia and stem cells

Later this month, the international stem cell research community will be travelling to Stockholm for the upcoming International Society for Stem Cell Research (ISSCR) meeting covering diverse topics from reprogramming and pluripotency of stem cells through tissue engineering and organ regeneration to therapy with stem cells.

Whitley H135 Hypoxystation
Whitley H135 Hypoxystation

Hypoxia is a crucial parameter determining the fate and development of stem cells, which leads Don Whitley Scientific to exhibit the Hypoxystation controlled environment workstation for low oxygen cell culture (see us on stand no. B15:33). Dr. Burga Kalz Fuller, Product Manager at our American distributor, HypOxygen, has summarized five recent papers delineating the role of hypoxia in stem cell research:

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Interrogating gynaecological cancer cell metabolism at different oxygen tensions reveals simvastatin as metabolic regulator.

Earlier this month at the Keystone Symposia, Hypoxia: From Basic Mechanisms to Therapeutics, Dr Ayse Latif (pictured below) from the University of Manchester presented a poster entitled “Interrogating gynaecological cancer cell metabolism at different oxygen tensions reveals simvastatin as metabolic regulator”.

The poster describes the background of the study
as follows:Ayse Latif Keystone

Around 200,000 new cases of gynaecological cancers are diagnosed in Europe every year. Potentially 75% of these cancers could benefit from improved treatment regimes. Gynaecological cancer cells have an increased glycolysis rate and lactate concentration which have been suggested to predict increased likelihood of metastasis, resistance to therapy and reduced survival in patients. Lactate transport in cancer cells is carried out by members of the monocarboxylate transporter (MCT) family, notably MCT1/4. Thus, we hypothesized that pharmacologic inhibition of MCTs could improve treatment outcome by reducing glycolytic potential of these tumour cells… (To continue reading, click here).

Researchers at the University of Manchester used a Whitley i2 Instrument Workstation, housing a Seahorse XF Analyzer, connected to a Whitley H35 HEPA Hypoxystation. This allowed for the preparation of cell lines under hypoxic conditions and their subsequent transfer to the i2 for analysis in a CO2 free and controlled temperature environment without exposure to ambient conditions.

 

 

 

H135 at Keystone Symposia: Dublin

Representatives from Don Whitley Scientific and our American distributor, HypOxygen, are attending the Keystone Symposia on Hypoxia: From Basic Mechanisms to Therapeutics this week in Dublin, Ireland. From May 12th – 17th you will have the opportunity to see the brand new Whitley H135 Hypoxic Workstation.

Delegates attending the Keystone Meeting can pick up a free Hypoxia t-shirt from the DWS stand - while stocks last!
Delegates attending the Keystone Meeting can pick up a free Hypoxia t-shirt from the DWS stand – while stocks last!

The largest workstation in our range, the H135 (pictured left) has an enormous capacity for working and incubation of over 550 litres. Combined with the ability to house large pieces of equipment such as live cell imaging devices, microscopes, plate readers and more – it’s our most revolutionary Hypoxystation yet.

Without compromising on quality or precision, this innovative product allows you to take your research to new heights whilst maintaining confidence in your results.

 Some of the great features include:

  • Rapid 12L airlock
  • 3 gas operation
  • Automated Ocalibration
  • Removable front
  • Option for Whitley Internal HEPA Filtration System with Enhanced Biological Containment

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Hypoxia and Immunity

Whitley H35 HypoxystationThe upcoming Cell symposium “Cancer, Inflammation and Immunity” will shine a spotlight on research delineating the complex cross-talk between inflammatory processes, immune response and the development of cancer diseases. Don Whitley Scientific will be exhibiting the Whitley H35 Hypoxystation (pictured right), a controlled environment workstation for low oxygen cell culture, at the meeting on June 14-16 in Sitges, Spain.

As we look forward to the conference, Dr Burga Kalz Fuller, Product Manager at our American distributor, HypOxygen, has summarized five interesting and recent papers concerning hypoxia and its role in immunology and cancer research:

1.         “A mechanism of hypoxia-mediated escape from adaptive immunity in cancer cells” Barsoum et al, Cancer Res. 2014 Feb 1;74(3):665-74

In cancer cells exposed to hypoxia, HIF-1α induced expression of programmed cell death ligand PD-L1, which increased the cells’ resistance to CTL-mediated lysis and contributed to tumoral immune escape. This effect was blocked through administration of glyceryl trinitrate (GTN), an agonist of nitric oxide signalling, suggesting that NO mimetica inhibiting PD-L1 may present a novel cancer therapy strategy.
2.         “HIF-mediated innate immune responses: cell signaling and therapeutic implications” Harris et al., Hypoxia 2014:2 47–58

Host defense through innate immune cells takes place in a low oxygen environment where functions as diverse as cytokine secretion and pathogen phagocytosis are modulated by HIF’s. This review summarises the roles of HIF’s in acute and chronic immune response and gives a perspective on therapies targeting the HIF pathway.

3.         “Identification of CD300a as a new hypoxia-inducible gene and a regulator of CCL20 and VEGF production by human monocytes and macrophages” Raggi et al., Innate Immunity October 2014 vol. 20 no. 7 721-734

Hypoxia is characteristic for sites of inflammation and lesion, and monocytes and other immune cells accumulating in these hypoxic areas are specifically stimulated by the low oxygen environment. Raggi et al. investigated the hypoxic transcriptome and describe members of the CD300 superfamily of immunoregulatory cell surface receptors which are up-regulated in hypoxia.

4.         “HIF Transcription Factors, Inflammation, and Immunity”, Palazon et al., Immunity 41, October 16, 2014

Hypoxia-signaling pathways which trigger HIF expression act in the immune system to modulate host immune function. In this review, Palazon et al. describe the myriad ways oxygen sensing regulates innate and adaptive immunity.

5.         “Hypoxia attenuates the proinflammatory response in colon cancer cells by regulating IκB”, Mueller-Edenborn, Oncotarget April 2015

Mueller-Edenborn’s group shed light on signalling pathways regulating hypoxia and inflammatory responses, which exhibit a surprising degree of cross-talk in colon cancer. Hypoxia attenuated proinflammatory responses by inhibiting translocation of NF-κB into the nucleus, demonstrating yet again that both these aspects of the tumour microenvironment influence therapy response.

H135: Taller, Deeper, Wider.

Don Whitley Scientific is proud to present the new Whitley H135 Hypoxystation; the tallest, deepest, widest workstation in our range.

This new hypoxic chamber has a usable internal volume of almost 600 litres and can accommodate a variety of items of equipment such as live cell imaging devices, microscopes, plate readers, etc. (Be sure to discuss your requirements with us at the earliest opportunity). The generous internal height also facilitates easy pipetting. All cell manipulations can be performed without removing them from your required hypoxic conditions.

With a volume twice that of a H35 Hypoxystation, the H135 also comes fitted with a large removable front, available with either two or three ports, plus the same great features and benefits as other Whitley Workstations, including:

  • Rapid 12 litre airlock

    H135 Hypoxystation
    H135 Hypoxystation
  • Three gas operation
  • The Whitley Internal HEPA Filtration System with Enhanced Biological Containment
  • The option of being connected to a Whitley i2 Instrument Workstation
  • 7” full colour touch screen that is Ethernet-enabled for remote access.

Internal dimensions (w x d x h) are 1100mm/43.3” x 750mm/29.5” x 710mm/28”.

External dimensions (w x d x h) are 1452mm/57.25″ x 1056mm/41.5″ x 993mm/39″.

Representatives from Don Whitley Scientific will be attending the Keystone Symposia in Dublin, 12-17 May, where you can be one of the first to see this revolutionary product.

Watch the introductory video here.