Clinical Microbiology of Anaerobes – Course Success

Don Whitley Scientific recently sponsored a two day residential course delivered by the UK Anaerobe Reference Unit, Public Health Wales. The Practical & Clinical Microbiology of Anaerobes Course was held on 25-26 June in Cardiff. The event served as a refresher on culturing, identifying and being aware of the clinical importance of anaerobes. 

The 20 places available on the course were filled within a few weeks of the event being promoted, proving that the subject matter is of real interest to today’s microbiologists. Delegates came from all over the UK: from Inverness to Truro!

The aims of the course were:

  • To promote an understanding and awareness of anaerobic bacteria in clinical material
  • To gain an insight into the relevance of anaerobic bacteria in clinical microbiology
  • For delegates to achieve a basic level of competence in methods used for the isolation and identification of anaerobes
  • To improve the standards of anaerobic microbiology in clinical laboratories.

Trefor Morris, Lead Scientist of the UKARU, said:

“I am very proud of the commitment and dedication of my team in delivering such an excellent course. The positive feedback shows that we must continue to use our expertise to reinforce the importance of anaerobic bacteria, particularly in the face of the challenges of antimicrobial resistance. Indeed the fact that the course is already almost full for next year is testament to the level of interest and its unique nature.”

“I am also particularly happy that the delegates found the course friendly and interesting highlighted by the comments below:”

“A friendly, knowledgeable and very interesting course that I would recommend to anyone.”

“Very friendly atmosphere throughout the course, very knowledgeable and helpful staff during practical.”

“Really enjoyed the course, excellently organised and learnt lots to take back to my laboratory.”

“A fantastic course, the human clinical case presentations were very interesting.”

 

Don Whitley Scientific arranged for the use of a Whitley A55 Anaerobic Workstation for the course.

 

A Don Whitley A55 Workstation
A Whitley A55 Anaerobic Workstation

Anaerobes. Where to Start?

Introduction to Clinical Anaerobic Bacteriology
Introduction to Clinical Anaerobic Bacteriology

To a newcomer, the field of anaerobic research can seem intimidatingly vast. With so many varieties of anaerobes, and differing means of culturing them, you may find yourself wondering whether the method you are using is really the most efficient and cost-effective.

The first thing you need to consider is the type of anaerobe you are cultivating, whilst aerotolerant organisms and facultative anaerobes will not be harmed by a small amount of oxygen, obligate anaerobes require a completely oxygen-free environment.

We have recently produced a useful reference guide: An Introduction to Clinical Anaerobic Bacteriology which enables the reader to isolate and identify 12 commonly occurring and clinically important anaerobic bacteria.

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Did You Know: Whitley Workstations are Ethernet-enabled for Remote Access?

Have you ever wanted to check the status of your Whitley Workstation when you were away from the lab? Well now you can. Most of our new workstations are Ethernet-enabled so you can dial in and access the touch-screen control panel from the other side of the building, or even from the other side of the world. This allows you to check the current operating conditions of the workstation and, if activated, view any of the graphical screens. If necessary, you can then make changes to the parameters, i.e. temperature, humidity, O2 and CO2 levels from wherever, whenever!

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Did You Know: The A55 is a Dual Temperature Workstation?

Whilst the Whitley A55 Anaerobic Workstation’s large capacity allows two people to work side-by-side in the same environmental conditions, this workstation is unique in our range as it can also be supplied with a central partition to separate the workstation into two chambers. This permits the user to select different temperature/humidity combinations for each side of the workstation.

Can I transfer samples between the two chambers internally?
A manually operated, sliding door within the partition allows for convenient sample transfer between the chambers.

What is the maximum temperature differential that can be achieved in each chamber?
A maximum temperature differential of approximately 8˚C can be achieved between the two chambers.

Can I restrict who can adjust the environmental conditions?

There are security levels in place to restrict who is authorised to adjust temperature and humidity.

How can I check the relative humidity inside the chamber?
The relative humidity inside each chamber, expressed as a percentage, is always displayed on the main screen.

For more information please contact us directly.

Did you know: How best to clean your workstation?

Laboratory Standard Shot DYK Blog

 

 

This is the 10th in our ‘Did you know’ series of articles introducing things you may not know about the Whitley range of products and services – read on and you may find out something about your workstation that could make your working life easier.

We regularly receive enquiries from customers with regard to which disinfectants can be safely used inside Whitley Workstations. Although it is not practicable to evaluate every available product, we have tested the materials compatibility of several sporicidal disinfectants and can now provide comprehensive advice on this topic.

 

 

How often should I clean my workstation?

DWS recommend that as part of the daily checks, users ensure that the workstation is free from spillage and cleaning carried out as necessary. Prior to having your workstation serviced, however, it may be necessary for it to be decontaminated.

Which disinfectants have DWS tested?

  • Spor-Klenz – peracetic acid (approx 0.1%) + hydrogen peroxide (approx 1.0%) – “ready to use” product, tested without further dilution.
  • Chemgene HLD4H – quaternary ammonium compounds (approx 1%) + chlorhexidine digluconate (approx 1%) – “ready to use” product, tested without further dilution.
  • Safe Hospital – hypochlorous acid – “ready to use” product, tested without further dilution.
  • Tristel Fuse – chlorine dioxide (approx 1%) – prepared from concentrated components according to manufacturer’s instructions.

For more information on these tests, please see the appendices of your Whitley Workstation user manual.

 

Which of the above products are OK for use in a Whitley Workstation?

Our tests demonstrated that each of these formulations is satisfactory for use in Whitley Workstations and will not cause any damage when used in accordance with the manufacturer’s recommendations.

 

What if I want to use a cleaning agent not on the above list?

Other disinfectants containing the active ingredients listed above, at similar concentrations to those shown, can also be safely used inside Whitley Workstations. If you wish to use a disinfectant containing active ingredients not listed here, please contact us for advice before proceeding.

 

Why should I contact DWS for advice – will any damage not be covered under my warranty?

Unfortunately, we cannot cover under the equipment’s warranty any damage caused to a Whitley Workstation as a result of exposure to products that have not been approved by us.

 

Can I use Virkon as my preferred sanitising solution?

After a thorough study lasting 20 weeks, we concluded that the presence of any uncovered containers of Virkon in anaerobic and variable atmosphere workstations leads to the degradation of stainless steel, brass and some other metal components. The effect is more considerable when oxygen is present in the selected gas mixture. These conclusions are supported by technical information available on the Virkon manufacturer’s website and independent observations by an Institute of Materials metallurgist. If Virkon is the preferred sanitising solution these adverse effects can be minimised by ensuring that Virkon within any workstation is always kept in a covered vessel when not in use.

 

OK, so what’s the best way to clean the acrylic parts of a Whitley Workstation?Labdet100 Blog

The transparent and/or white acrylic on the inside and outside of the Whitley Workstation System may be swabbed with a 2% solution of Labdet 100 (DWS – stock code D00003) in warm water and dried afterwards with a soft clean cloth. In the case of culture spillages then any of the disinfectants listed above solution should be applied to the spillage and left for 30 minutes. It should then be mopped.

 

Never use any solvent on the acrylic surfaces of the workstation. Use only water and a mild detergent solution (i.e. Labdet 100 2% solution) as a cleaning agent.

 

What if the spillage leaks underneath the floor of the workstation?

If spillage is not contained on the working surface then access underneath this area will be required in all Whitley Workstations except the DG250. Some workstations require the removal of retaining bolts before the floor can be lifted up or pivoted for cleaning underneath.

If liquid has been spilled upon the Anotox and catalyst sachets they should be removed, dried and/or replaced.

 

How can I deal with scratches on the acrylic plastic surface of my workstation?

Scratches may be removed by gently polishing the surface with ‘DURAGLIT WADDING’ followed by wiping with a soft clean cloth. Deep scratches may require the use of Wet and Dry abrasive paper used wet, followed by polishing with ‘DURAGLIT’ – seek advice from Don Whitley Scientific Limited or our authorised agents overseas.

 

How can I clean the sleeves on my workstation?Sleeve Kit-Components DYK Blog

The white sleeves used on all Whitley Workstations may require cleaning after periods of extended use. There are a number of options for cleaning and some recommendations listed below:

  • Wipe with a mild detergent
  • Wash or dry-clean with a detergent up to 75ºC
  • For disinfection use Sodium Hypochlorite (at no more than 1000ppm active Chlorine) or any of the disinfectants listed above
  • Do not use Biological or Phenolic based cleaners
  • Wash and dry completely before storage
  • Some surface wrinkling may occur.

 

If you have any remaining concerns about disinfectants for use in Whitley Workstations, please get in touch with our sales team:

Tel: 01274 595728

Or

Email: sales@meintrup-dws.de

 

A55 Workstation Installed at Southern General

Southern General Glasgow A55 Installation

This is a photograph of a Whitley A55 Anaerobic Workstation that was installed last week at Southern General Hospital in Glasgow. The picture shows (from left to right) Gordon Mackie of Southern General’s Microbiology Department with installation team Adam Murray and Joe Walton.

 

New 2-Airlock Workstation – European Launch

Biotechnica 2013

Are you attending Biotechnica in Hannover this year?

Would you like to see the latest in workstation technology?

 

Whitley A55 Workstation

Come along to the European launch of the Whitley A55 Anaerobic Workstation at Biotechnica, 8th – 10th October 2013.

Also on show will be the Rapid Automated Bacterial Impedance Technique (RABIT), Whitley Jar Gassing System, Whitley Automated Spiral Plater (WASP), the new TEB500 bioreactor and a range of water activity meters.

We would be delighted if you would call at stand number C75/1 in Hall 9 to meet us and our German subsidiary company, Meintrup-DWS Laborgeräte. If you would like an appointment at the show with our Export Manager, please email us on sales@meintrup-dws.de.

We look forward to seeing you there.

Workstation with two airlocks launched

Whitley A55 Workstation

Don Whitley Scientific Limited (DWS) has launched a new 4-port anaerobic workstation that has two airlocks, one at either end of the chamber. Unveiled at the Biomedical Science Congress in Birmingham in September, this workstation has been designed for busy laboratories where two people may need to use the workstation simultaneously. The positioning of the two airlocks at either end of the chamber allows samples to be introduced at one end and removed at the other, significantly improving laboratory workflow.

The A55 incorporates all the latest Whitley Workstation features such as a colour touchscreen interface, sleeveless instant access ports, automatic commissioning and a range of options and accessories to tailor the workstation to the customer’s requirements. A removable front is fitted as standard on the right hand side of the cabinet to facilitate the introduction of bulk samples and equipment that may need to be used inside the chamber. The A55 can accommodate up to 1,400 x 90mm Petri dishes.

Evan Kitsell, Design Director, commented: “Our research into customer requirements confirmed that there was an need for a larger workstation with an airlock at either end. We discovered that the increased throughput in some laboratories has led to a need for improved workflow, larger cabinets and a the ability for additional staff to work simultaneously in the chamber.”

Options for the A55 include a fully integrated Anaerobic Conditions Monitor, internal power sockets, single plate entry system and a fully comprehensive maintenance contract.