Huge Capacity with the A95TG

The Whitley A95TG (three gas) Anaerobic Workstation is currently the largest model in our range, with a maximum capacity of between 1000 and 1400 x 90mm Petri dishes. It is also extremely cost-effective; with an operating cost saving of 30% compared to the dual gas option.

The large, integral 30 litre airlock with automatic internal door is 20% faster than our previous MG1000 model, with the cycle taking just 5 minutes.

A95 Airlock Open2

Each porthole also acts as a mini-airlock so you can transfer 10 plates through each time you enter or exit the workstation, thereby reducing unnecessary airlock cycles when processing small sample quantities.


Ideal for use in teaching hospitals and university laboratories, the four access ports allow two users to work side-by-side.

Available with fully integrated Anaerobic Conditions Monitor with data download facility, other options include: internal power sockets, bespoke trolley and single plate entry systems.

For more details, please contact us.

Anaerobes. Where to Start?

Introduction to Clinical Anaerobic Bacteriology
Introduction to Clinical Anaerobic Bacteriology

To a newcomer, the field of anaerobic research can seem intimidatingly vast. With so many varieties of anaerobes, and differing means of culturing them, you may find yourself wondering whether the method you are using is really the most efficient and cost-effective.

The first thing you need to consider is the type of anaerobe you are cultivating, whilst aerotolerant organisms and facultative anaerobes will not be harmed by a small amount of oxygen, obligate anaerobes require a completely oxygen-free environment.

We have recently produced a useful reference guide: An Introduction to Clinical Anaerobic Bacteriology which enables the reader to isolate and identify 12 commonly occurring and clinically important anaerobic bacteria.


Did You Know: Whitley Workstations are Ethernet-enabled for Remote Access?

Have you ever wanted to check the status of your Whitley Workstation when you were away from the lab? Well now you can. Most of our new workstations are Ethernet-enabled so you can dial in and access the touch-screen control panel from the other side of the building, or even from the other side of the world. This allows you to check the current operating conditions of the workstation and, if activated, view any of the graphical screens. If necessary, you can then make changes to the parameters, i.e. temperature, humidity, O2 and CO2 levels from wherever, whenever!


New Chilled Incubation Compartment

If you need to store small quantities of media and samples in anaerobic conditions, but at lower than ambient temperatures, you can now specify an optional Chilled Incubation Compartment for a Whitley A35 or A45 Workstation.

This insulated compartment is located on the left hand side of the workstation and is accessed from the main chamber. When you open the compartment door, the plate carrier basket swings outwards to allow easy access. It has a capacity of 10 x 90mm Petri dishes or items of a similar size.

The temperature in the Chilled Incubation Compartment can be easily adjusted, in 0.1°C increments, via the workstation’s colour touchscreen. The operating temperature range is between +12°C and +25°C and the temperature distribution within the compartment is within 2°C*.

This option must be specified at time of order as it is a factory-fitted modification.


*Laboratory and main chamber temperatures may influence the upper and lower temperature levels that can be achieved.

Did you know: How best to clean your workstation?

Laboratory Standard Shot DYK Blog



This is the 10th in our ‘Did you know’ series of articles introducing things you may not know about the Whitley range of products and services – read on and you may find out something about your workstation that could make your working life easier.

We regularly receive enquiries from customers with regard to which disinfectants can be safely used inside Whitley Workstations. Although it is not practicable to evaluate every available product, we have tested the materials compatibility of several sporicidal disinfectants and can now provide comprehensive advice on this topic.



How often should I clean my workstation?

DWS recommend that as part of the daily checks, users ensure that the workstation is free from spillage and cleaning carried out as necessary. Prior to having your workstation serviced, however, it may be necessary for it to be decontaminated.

Which disinfectants have DWS tested?

  • Spor-Klenz – peracetic acid (approx 0.1%) + hydrogen peroxide (approx 1.0%) – “ready to use” product, tested without further dilution.
  • Chemgene HLD4H – quaternary ammonium compounds (approx 1%) + chlorhexidine digluconate (approx 1%) – “ready to use” product, tested without further dilution.
  • Safe Hospital – hypochlorous acid – “ready to use” product, tested without further dilution.
  • Tristel Fuse – chlorine dioxide (approx 1%) – prepared from concentrated components according to manufacturer’s instructions.

For more information on these tests, please see the appendices of your Whitley Workstation user manual.


Which of the above products are OK for use in a Whitley Workstation?

Our tests demonstrated that each of these formulations is satisfactory for use in Whitley Workstations and will not cause any damage when used in accordance with the manufacturer’s recommendations.


What if I want to use a cleaning agent not on the above list?

Other disinfectants containing the active ingredients listed above, at similar concentrations to those shown, can also be safely used inside Whitley Workstations. If you wish to use a disinfectant containing active ingredients not listed here, please contact us for advice before proceeding.


Why should I contact DWS for advice – will any damage not be covered under my warranty?

Unfortunately, we cannot cover under the equipment’s warranty any damage caused to a Whitley Workstation as a result of exposure to products that have not been approved by us.


Can I use Virkon as my preferred sanitising solution?

After a thorough study lasting 20 weeks, we concluded that the presence of any uncovered containers of Virkon in anaerobic and variable atmosphere workstations leads to the degradation of stainless steel, brass and some other metal components. The effect is more considerable when oxygen is present in the selected gas mixture. These conclusions are supported by technical information available on the Virkon manufacturer’s website and independent observations by an Institute of Materials metallurgist. If Virkon is the preferred sanitising solution these adverse effects can be minimised by ensuring that Virkon within any workstation is always kept in a covered vessel when not in use.


OK, so what’s the best way to clean the acrylic parts of a Whitley Workstation?Labdet100 Blog

The transparent and/or white acrylic on the inside and outside of the Whitley Workstation System may be swabbed with a 2% solution of Labdet 100 (DWS – stock code D00003) in warm water and dried afterwards with a soft clean cloth. In the case of culture spillages then any of the disinfectants listed above solution should be applied to the spillage and left for 30 minutes. It should then be mopped.


Never use any solvent on the acrylic surfaces of the workstation. Use only water and a mild detergent solution (i.e. Labdet 100 2% solution) as a cleaning agent.


What if the spillage leaks underneath the floor of the workstation?

If spillage is not contained on the working surface then access underneath this area will be required in all Whitley Workstations except the DG250. Some workstations require the removal of retaining bolts before the floor can be lifted up or pivoted for cleaning underneath.

If liquid has been spilled upon the Anotox and catalyst sachets they should be removed, dried and/or replaced.


How can I deal with scratches on the acrylic plastic surface of my workstation?

Scratches may be removed by gently polishing the surface with ‘DURAGLIT WADDING’ followed by wiping with a soft clean cloth. Deep scratches may require the use of Wet and Dry abrasive paper used wet, followed by polishing with ‘DURAGLIT’ – seek advice from Don Whitley Scientific Limited or our authorised agents overseas.


How can I clean the sleeves on my workstation?Sleeve Kit-Components DYK Blog

The white sleeves used on all Whitley Workstations may require cleaning after periods of extended use. There are a number of options for cleaning and some recommendations listed below:

  • Wipe with a mild detergent
  • Wash or dry-clean with a detergent up to 75ºC
  • For disinfection use Sodium Hypochlorite (at no more than 1000ppm active Chlorine) or any of the disinfectants listed above
  • Do not use Biological or Phenolic based cleaners
  • Wash and dry completely before storage
  • Some surface wrinkling may occur.


If you have any remaining concerns about disinfectants for use in Whitley Workstations, please get in touch with our sales team:

Tel: 01274 595728




Consistent growth. Just what you need for your anaerobes.

Then and Now

From our first workstation nearly 40 years ago, our product range continues to grow and grow.

Our latest enterprise, the A95 Anaerobic Workstation – as featured in the official IBMS 2015 diary – has four unique gloveports that double as sample transfer systems, a huge incubation capacity, a large integral airlock, an intuitive full-colour 7” touchscreen, network connectivity, data downloading and anaerobic conditions monitoring.

For more information please contact us:


t: +44 (0)1274 595728.

Hypoxystations now with superior cooling

Whitley H35 Hypoxystation

An example of how quickly Don Whitley Scientific can react to customer needs came about recently due to a query from our Chinese distributor.

A Hypoxystation customer was experiencing problems with overnight humidity control in the workstation. They explained that most laboratories in China are air conditioned during the day but the air conditioning is usually switched off outside office hours. Customers were finding that because of this, the ambient temperature in the laboratory was rising to such a level that effective cooling of the condenser plate could not be maintained. Although this may not be an issue when working with human cells, this customer was using the H35 Hypoxystation for anaerobic organisms.

Our distributor, Hua Yue Enterprise Holdings, asked if there was anything that could be done to improve the cooling system so that switching off the air conditioning overnight had less of an effect on the internal atmosphere of the workstation.

We set to work on the challenge, acknowledging that this issue could apply to any laboratory in any country where air conditioning systems are used in laboratories – not something we usually have to worry about in the UK!

Our research and development team had already improved Whitley Anaerobic Workstations by fitting them with thermoelectric humidity control devices for cooling the condenser plate. This has proved to be a much more efficient method of cooling.

This improved control of humidity has been so successful, it seemed only sensible to fit this superior cooling device to all our H35 and H45 Hypoxystations, including the HEPA models.

This change came into effect at the beginning of July … the first unit being supplied to China, of course.

And the winner is … Antibiotics

Whitley H35 Anaerobic Workstation

For those who are not already aware, the 2014 Longitude Prize challenge is to create a cost-effective, accurate, rapid and easy-to-use test kit for bacterial infections that will allow health professionals to administer the right antibiotics at the right time. Scientists now have 5 years in which to come up with a solution and potentially claim the £10m prize fund.


A35 Used in Clostridium difficile Study

Flock G and Mooyottu S at ASM 2014

A poster entitled: ‘Efficacy of Octenidine Hydrochloride in Reducing Hyper-virulent Clostridium difficile Spores on Stainless Steel Surface’ was presented at the American Society for Microbiology, held in Boston (May 2014). The authors, Genevieve Flock, Shankumar Mooyottu and Kumar Venkitanarayanan, from the Department of Animal Science, University of Connecticut, had used a Whitley A35 Anaerobic Workstation in their study. Here’s a photograph of Genevieve and Shankumar at the ASM with their poster.

A total of four research abstracts from the lab, including an award winning poster on C. difficile epidemiology and pathogenesis, in which the Whitley A35 Workstation was used as an integral part of the research, were presented at ASM 2014 and at IFT (Institute of Food Technologists) 2013.

A manuscript entitled: ‘Carvacrol and trans-Cinnamaldehyde Reduce Clostridium difficile Toxin Production and Cytotoxicity in Vitro’, published this year, can be found on the Don Whitley Scientific website.

Poster and photograph reproduced by kind permission of Genevieve Flock.

NEW Spotlight accessory for Whitley H85, A85 and A95 Workstations

Workstation spotlight

Just to let you know that our range of spotlight assemblies for Whitley Workstations has been extended. We now offer this option for the A85 Workstation, H85 Hypoxystation and A95 Workstation. Spotlights typically provide directional lighting to assist in the early identification of sample growth or other specimen examination.