New Poster from Liverpool University

Researchers from the University of Liverpool (Department of Infection Biology, Institute of Infection & Global Health and School of Veterinary Science), have produced a poster entitled “Understanding the aetiology and infection reservoirs of digital dermatitis in beef cattle and sheep”.

As part of their work, they cultured treponemes in a Whitley A35 Anaerobic Workstation.

Whitley A35 Anaerobic Workstation
Whitley A35 Anaerobic Workstation

Abstract:

Digital dermatitis (DD) is an infectious hoof disease causing severe lameness in ruminants worldwide. The primary cause has been identified as the spirochaetal bacteria, treponemes. A comprehensive bacterial molecular survey of sheep contagious ovine digital dermatitis (CODD) lesions has not been done to determine if there is a shared etiopathogenesis between BDD and CODD. Also, there have been only anecdotal reports of BDD lesions occurring in beef cattle, with no definitive published data on the disease.  Additionally, little is known about the transmission of Treponema bacteria. It may be possible that DD treponemes are carried in both cattle and sheep gastrointestinal (GI) tracts and then shed in faeces and spread. Another route of transmission may be via farm equipment.

This study investigated ruminant GI tissues and farm equipment for the presence of DD treponemes. Additionally, sheep and beef DD lesions were analysed for the presence of DD treponemes by PCR and culture techniques.

The full poster can be viewed  here.

New Protos 3 Colony Counter

New Protos 3 colony counter and rapid ID system saves time and provides accurate, traceable results in microbiology

Don Whitley Scientific Limited introduces a vibrant new automated colony counting and chromogenic identification system, Protos 3. This system allows accurate and reproducible identification of colonies cultured on chromogenic plates.

What makes the new Protos 3 outperform other commercial colony counters is the system’s ability to count colonies in seconds and identify microbial species by their colour on chromogenic plates. This is a great time saver, providing accurate, objective and fully traceable GLP compliant results.

The stylish, yet practical Protos 3, which comes in bright red, attaches easily to a computer and requires minimal training to set up. Users simply input their plate identification and click. The Protos 3, featuring a highly sensitive CCD camera combined with unique three colour LED lighting, rapidly images an infinite number of colony colours on one plate and detects colonies as small as 0.043mm.

The Protos 3’s powerful software then generates true to life counts and plate images, which can be transferred and stored in Excel. This GLP compliant process, with its full audit trail eliminates keying and image transfer errors providing accurate, objective data, which can be reviewed anywhere and anytime.

“Microbiologists have always wanted a colony counter that combines throughput and automatic colony ID,” commented Steve Robertson, Sales Director. “The new Protos 3 offers a high performance platform that generates fully traceable data and we know it will be a huge time saving device for microbiologists in food and pharmaceutical laboratories. For anyone using a spiral plater for carrying out multiple bacterial enumerations, this is a perfect solution.” 

Botulism Cases Rise

Scottish media has recently reported an increase in the number of diagnosed cases of botulism. Caused by toxins produced by the bacterium Clostridium botulinum, this condition attacks the nervous system, can cause paralysis, and may be fatal if left untreated. There are three types of botulism: food-borne, wound and infant. Diagnosis is achieved by conducting tests on samples of blood, faeces, stomach contents or food to detect the bacteria or toxins.

Having to send samples off to an external laboratory for analysis can mean delaying diagnosis and by extension, treatment, for longer than necessary. Hospitals which are equipped to perform these tests in-house will have a much greater chance of containing and treating this illness effectively.

Anaerobic jars can be restrictive when processing a large number of samples, whereas an anaerobic workstation provides a sustainable environment where parameters can be altered to create the required conditions.

The Whitley A35 Anaerobic Workstation can accommodate up to 600 x 90mm Petri dishes and like our other anaerobic chambers, it provides excellent conditions for the processing, incubation and examination of samples without exposure to atmospheric oxygen.

For more information please contact us.

Anaerobes. Where to Start?

Introduction to Clinical Anaerobic Bacteriology
Introduction to Clinical Anaerobic Bacteriology

To a newcomer, the field of anaerobic research can seem intimidatingly vast. With so many varieties of anaerobes, and differing means of culturing them, you may find yourself wondering whether the method you are using is really the most efficient and cost-effective.

The first thing you need to consider is the type of anaerobe you are cultivating, whilst aerotolerant organisms and facultative anaerobes will not be harmed by a small amount of oxygen, obligate anaerobes require a completely oxygen-free environment.

We have recently produced a useful reference guide: An Introduction to Clinical Anaerobic Bacteriology which enables the reader to isolate and identify 12 commonly occurring and clinically important anaerobic bacteria.

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New Chilled Incubation Compartment

If you need to store small quantities of media and samples in anaerobic conditions, but at lower than ambient temperatures, you can now specify an optional Chilled Incubation Compartment for a Whitley A35 or A45 Workstation.

This insulated compartment is located on the left hand side of the workstation and is accessed from the main chamber. When you open the compartment door, the plate carrier basket swings outwards to allow easy access. It has a capacity of 10 x 90mm Petri dishes or items of a similar size.

The temperature in the Chilled Incubation Compartment can be easily adjusted, in 0.1°C increments, via the workstation’s colour touchscreen. The operating temperature range is between +12°C and +25°C and the temperature distribution within the compartment is within 2°C*.

This option must be specified at time of order as it is a factory-fitted modification.

 

*Laboratory and main chamber temperatures may influence the upper and lower temperature levels that can be achieved.

Did you know: How best to clean your workstation?

Laboratory Standard Shot DYK Blog

 

 

This is the 10th in our ‘Did you know’ series of articles introducing things you may not know about the Whitley range of products and services – read on and you may find out something about your workstation that could make your working life easier.

We regularly receive enquiries from customers with regard to which disinfectants can be safely used inside Whitley Workstations. Although it is not practicable to evaluate every available product, we have tested the materials compatibility of several sporicidal disinfectants and can now provide comprehensive advice on this topic.

 

 

How often should I clean my workstation?

DWS recommend that as part of the daily checks, users ensure that the workstation is free from spillage and cleaning carried out as necessary. Prior to having your workstation serviced, however, it may be necessary for it to be decontaminated.

Which disinfectants have DWS tested?

  • Spor-Klenz – peracetic acid (approx 0.1%) + hydrogen peroxide (approx 1.0%) – “ready to use” product, tested without further dilution.
  • Chemgene HLD4H – quaternary ammonium compounds (approx 1%) + chlorhexidine digluconate (approx 1%) – “ready to use” product, tested without further dilution.
  • Safe Hospital – hypochlorous acid – “ready to use” product, tested without further dilution.
  • Tristel Fuse – chlorine dioxide (approx 1%) – prepared from concentrated components according to manufacturer’s instructions.

For more information on these tests, please see the appendices of your Whitley Workstation user manual.

 

Which of the above products are OK for use in a Whitley Workstation?

Our tests demonstrated that each of these formulations is satisfactory for use in Whitley Workstations and will not cause any damage when used in accordance with the manufacturer’s recommendations.

 

What if I want to use a cleaning agent not on the above list?

Other disinfectants containing the active ingredients listed above, at similar concentrations to those shown, can also be safely used inside Whitley Workstations. If you wish to use a disinfectant containing active ingredients not listed here, please contact us for advice before proceeding.

 

Why should I contact DWS for advice – will any damage not be covered under my warranty?

Unfortunately, we cannot cover under the equipment’s warranty any damage caused to a Whitley Workstation as a result of exposure to products that have not been approved by us.

 

Can I use Virkon as my preferred sanitising solution?

After a thorough study lasting 20 weeks, we concluded that the presence of any uncovered containers of Virkon in anaerobic and variable atmosphere workstations leads to the degradation of stainless steel, brass and some other metal components. The effect is more considerable when oxygen is present in the selected gas mixture. These conclusions are supported by technical information available on the Virkon manufacturer’s website and independent observations by an Institute of Materials metallurgist. If Virkon is the preferred sanitising solution these adverse effects can be minimised by ensuring that Virkon within any workstation is always kept in a covered vessel when not in use.

 

OK, so what’s the best way to clean the acrylic parts of a Whitley Workstation?Labdet100 Blog

The transparent and/or white acrylic on the inside and outside of the Whitley Workstation System may be swabbed with a 2% solution of Labdet 100 (DWS – stock code D00003) in warm water and dried afterwards with a soft clean cloth. In the case of culture spillages then any of the disinfectants listed above solution should be applied to the spillage and left for 30 minutes. It should then be mopped.

 

Never use any solvent on the acrylic surfaces of the workstation. Use only water and a mild detergent solution (i.e. Labdet 100 2% solution) as a cleaning agent.

 

What if the spillage leaks underneath the floor of the workstation?

If spillage is not contained on the working surface then access underneath this area will be required in all Whitley Workstations except the DG250. Some workstations require the removal of retaining bolts before the floor can be lifted up or pivoted for cleaning underneath.

If liquid has been spilled upon the Anotox and catalyst sachets they should be removed, dried and/or replaced.

 

How can I deal with scratches on the acrylic plastic surface of my workstation?

Scratches may be removed by gently polishing the surface with ‘DURAGLIT WADDING’ followed by wiping with a soft clean cloth. Deep scratches may require the use of Wet and Dry abrasive paper used wet, followed by polishing with ‘DURAGLIT’ – seek advice from Don Whitley Scientific Limited or our authorised agents overseas.

 

How can I clean the sleeves on my workstation?Sleeve Kit-Components DYK Blog

The white sleeves used on all Whitley Workstations may require cleaning after periods of extended use. There are a number of options for cleaning and some recommendations listed below:

  • Wipe with a mild detergent
  • Wash or dry-clean with a detergent up to 75ºC
  • For disinfection use Sodium Hypochlorite (at no more than 1000ppm active Chlorine) or any of the disinfectants listed above
  • Do not use Biological or Phenolic based cleaners
  • Wash and dry completely before storage
  • Some surface wrinkling may occur.

 

If you have any remaining concerns about disinfectants for use in Whitley Workstations, please get in touch with our sales team:

Tel: 01274 595728

Or

Email: sales@meintrup-dws.de

 

Practical Guide to Clinical Anaerobic Bacteriology

Introduction to Clinical Anaerobic Bacteriology

Eighteen months ago, Don Whitley Scientific played an integral role in producing An Introduction to Clinical Anaerobic Bacteriology. This unique guide is being very well-received by scientists in laboratories nationwide. It enables the reader to isolate and identify 12 commonly occurring clinically important anaerobic bacteria. It contains 60 pages printed on waterproof, tear-resistant synthetic paper to withstand regular use at the bench and is illustrated with stunningly detailed colour photographs and attractive reference tables.

Designed to fill a gap in the practical reference materials currently available to support clinical laboratory practice for microbiologists, the publication was written by Professor Michael W D Wren, MBE FIBMS, former consultant Biomedical Scientist in the microbiology department, University College Hospital, and visiting Professor at The University of Westminster. With a foreword by Professor Brian Duerden, Emeritus Professor of Medical Microbiology at Cardiff University and input from Dr Don Whitley, Chairman and founder of Don Whitley Scientific Limited, considerable expert knowledge has been combined to make this the most up-to-date reference guide for the modern clinical laboratory.

Images were kindly supplied by the Anaerobe Reference Unit, Cardiff.

For further information, or to order your copy, please contact us at sales@meintrup-dws.de or call us on 01274 595728.

 

Practical Guide to Clinical Anaerobic Bacteriology Launched

Anaerobic Reference Guide Book

Anaerobic microbiology specialist, Don Whitley Scientific (DWS) has launched a new publication ‘An Introduction to Clinical Anaerobic Bacteriology. This guide enables the reader to isolate and identify 12 commonly occurring clinically important anaerobic bacteria. It contains 60 pages printed on waterproof, tear-resistant synthetic paper to withstand regular use at the bench and is illustrated with stunningly detailed colour photographs and attractive reference tables.

(more…)