HEPA-Filtration von Don Whitley Scientific

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Seit über 40 Jahren nutzt Don Whitley Scientific Erfahrung und Know-how, um sicherzustellen, dass die Produkte die Arbeitsmethoden für unsere Laborkunden einfacher machen. Da immer mehr Wert auf saubere Luft gelegt wird, kann die HEPA-Filtration eine zusätzliche Maßnahme sein, um sicherzustellen, dass die in den Whitley Arbeitsstationen durchgeführten Untersuchungen in einer Umgebung durchgeführt werden, die Partikelkonzentrationen über den in ISO 14644-1 Teil 1, Tabelle 1, angegebenen Werten erreicht. (Klasse 3) Klassifizierung der Luftreinheit.

 

Don Whitley Scientific hat Tests durchgeführt, die zeigen, welchen Unterschied ein HEPA-Filtersystem zur Atmosphäre einer Whitley Workstation macht. Don Whitley Scientific stellt seit über 40 Jahren Produkte her und die Einführung der HEPA-Filtration war ein logischer Schritt, um sicherzustellen, dass unsere Kunden eine Option haben, die den neuesten Labortrends und -anforderungen entspricht.

 

Die Vorteile des Whitley HEPA Filtersystems sind:

  • Das Whitley-System verwendet einen internen HEPA-Filter. Die Atmosphäre wird schnell und kontinuierlich gereinigt, da das gesamte interne atmosphärische Volumen jeder Whitley Workstation den Filter hunderte Male pro Stunde durchläuft.
  • Die Atmosphäre, die durch den Filter strömt, erzeugt einen laminaren, strömungsartigen Effekt, der etwa 94% der Breite der Kammer abdeckt.
  • Der Standort des Whitley HEPA-Filters innerhalb der Arbeitsstation stellt sicher, dass es nicht nass werden kann. Daher kann es nicht mit Feuchtigkeit gesättigt und unwirksam gemacht werden.

Don Whitley Scientific hat in Zusammenarbeit mit einem der weltweit führenden Filterhersteller einen Filter entwickelt, der speziell auf die Anforderungen anaerober Arbeitsstationen zugeschnitten ist. Durch Hinzufügen der HEPA-Filtertechnologie zur Arbeitsstation wird eine Umgebung geschaffen, in der mikrobiologische Proben und Zellkulturen frei von Kontaminationsrisiken wachsen können.

 

HEPA-Filtration wurde als Option nach jahrzehntelanger Erfahrung in der Mikrobiologie eingeführt. Wir sind zuversichtlich, dass unsere gründliche Herangehensweise an die Produktentwicklung und Produktprüfung zu einem HEPA-Filtersystem geführt hat, das allen anderen Systemen überlegen ist, die an Arbeitsplätzen mit positivem Druck und modifizierter Atmosphäre installiert sind. Das Video unten erklärt die Whitley HEPA-Filtration in weiteren Einzelheiten.

 

 

 

Entdecken Sie die Whitley HEPA Workstation Reihe.

 

Whitley H35 Hypoxystation used in heart regeneration project

H35 Australia 1

Dr. Vaibhao Janbandhu is a Postdoctoral Research Scientist at the Victor Chang Cardiac Research Institute (VCCR) in Sydney. He has been in contact with Don Whitley Scientific to explain how his lab’s work has benefited from the use of a Whitley H35 Hypoxystation. Vaibhao uses the Hypoxystation to isolate, culture and characterise adult cardiac stem cells (CSCs).

 

Dr. Janbandhu had already been using a H35 that was set up at the institute for almost three years before he got his own unit installed last year. Specifically, his project is to find new ways to stimulate heart regeneration during ageing and after heart attack. For this he needs a way to isolate, culture and characterise adult CSCs. In Vaibhao’s words the H35 Hypoxystation seems well suited for this application: “the DWS Hypoxystation provides a highly stabilised HEPA-filtered environment in which levels of oxygen, carbon dioxide, temperature and humidity are precisely controlled and it will be an integral part of the project to advance the project aims”.

 

Vaibhao explains that mammalian stem cells reside in a specialised cellular microenvironment. This niche and the stem cell niche is characterised by a low partial oxygen pressure. This hypoxic niche protects stem cells from deleterious effects of O2 on proteins and DNA. These precise conditions are essential for Dr. Janbandhu’s work as they accurately replicate the in vivo environment.

 

His work also see benefits when comparing the use of a Hypoxystation as opposed to using a CO2 incubator. Typically, cell culture work involves methods which include isolating cells under their usual physiologically relevant conditions and then working with them in “bench-top conditions” where cells are exposed to non-physiological oxygen. This can then lead to altered hypoxic response, metabolism, reactive oxygen species and DNA damage response. This metabolic stress introduces unknown outcomes and may lead to results inconsistent with physiological processes. Therefore, the precise control of oxygen levels in cell culture has been shown to be vital for reproducible and physiologically relevant results, transforming the working environment in Vaibhao’s lab.

 

As well as the precise controlling of conditions, Vaibhao likes the remote access feature on his H35 Hypoxystation. The remote access allows Vaibhao to log into his Hypoxystation’s touchscreen control whilst away from the unit, offering increased flexibility in his working methods. Additionally, he likes how he can view operating conditions, set parameters and change access levels remotely.

 

Dr. Janbandhu opted to purchase a Hypoxystation from Don Whitley Scientific for the level of service and specification we were able to provide in Australia. Other companies either couldn’t fulfil configuration requirements and didn’t provide sales and or service in Australia. Don Whitley Scientific’s office in Australia, provides both fantastic sales and service nationwide. Vaibhao also states that from “discussion with other research groups across the world we felt confident to go for a DWS Hypoxystation”.

 

Dr. Vaibhao Janbandhu has this to say about Don Whitley Scientific Pty Ltd: “I would like to thank your company personnel at the customer services division in Australia for their excellent support. Your Sales & Service Manager in Australia, Grant Shallcross, took care of all my queries in a jiffy!”. Vaibhao added that the funding for the purchase came from the James N Kirby Foundation and the Victor Chang Cardiac Research Institute.

 

H35 Australia 2

i2 and H35 HEPA at ICR London

Whitley i2 Instrument Workstation used in deciphering the metabolic properties of Breast Cancer

The Institute of Cancer Research, London, is Europe’s largest cancer centre and one of the world’s most influential research institutes, with an outstanding record of achievement dating back more than 100 years.

Staff at the ICR with their new Whitley Workstations
ICR staff with their i2 and H35 HEPA Workstations

Cancer cells display unique alterations in their signalling and metabolic circuitry in order to fuel their growth and proliferation and/or adapt to conditions of oxygen and nutrient deprivation. The Signalling and Cancer Metabolism Team, led by Dr George Poulogiannis uses high-throughput technologies including mass spectrometry-based metabolomics, and utilizes the Seahorse XF Analyzer to measure the basal oxygen consumption and glycolysis rates in order to reveal the metabolic dependencies of breast cancer-driven alterations. We are using a Whitley i2 Instrument Workstation to study the signalling and metabolic pathways that may preserve breast cancer cell viability under hypoxia. In addition to this, we have a general interest in identifying what signalling and metabolic pathways are associated with the acquisition of microsatellite (MSI) and chromosomal instability (CIN) under oxygen deprivation conditions. Finally, we are studying the role of hypoxia in cell invasion and metastasis, oncogene-induced senescence and resistance to current treatment options.

George Poulogiannis
Team Leader
Division of Cancer Biology
Institute of Cancer Research, London

i2 Workstation Testimonial Video

In this video – the latest in our series of customer testimonials – Dr Ayse Latif, Faculty of Medical and Human Sciences, University of Manchester, discusses her current research of exploiting tumour cell energy metabolism in order to improve gynaecological cancer treatments.

Dr Latif uses both a H35 HEPA Hypoxystation and an i2 Instrument Workstation. Whilst cells are initially incubated in the H35, they are then moved into the i2 via the Whitley Transfer Tunnel without exposure to ambient conditions, where they can be studied with a Seahorse XF Analyzer.

The i2 was designed to meet the exact requirements  of Seahorse Bioscience, meaning that the combination of this workstation and a Seahorse XF Analyzer permits simultaneous, real-time analysis of mitochondrial respiration and glycolysis in mammalian cells under precisely controlled conditions.

Did You Know: Whitley Workstations are Ethernet-enabled for Remote Access?

Have you ever wanted to check the status of your Whitley Workstation when you were away from the lab? Well now you can. Most of our new workstations are Ethernet-enabled so you can dial in and access the touch-screen control panel from the other side of the building, or even from the other side of the world. This allows you to check the current operating conditions of the workstation and, if activated, view any of the graphical screens. If necessary, you can then make changes to the parameters, i.e. temperature, humidity, O2 and CO2 levels from wherever, whenever!

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Biological Containment

Whitley H35 Hypoxystation

Looking for extra biological containment from your workstation?

If you need additional biological containment within your HEPA filtered Workstation, there is now an option to reduce biological discharge from the chamber via the gas outlets. The Whitley Enhanced Biological Containment System involves fitting HEPA filters to the two gas outlets on a Whitley HEPA Workstation.

Laboratory tests were conducted on an H35 Hypoxystation to deliberately challenge the exhaust filters with high concentrations of aerosolized bacterial cells and quantify their discharge through the exhaust valve filters.

The results of this experiment demonstrated that the system achieved a substantial reduction in bacterial loading (better than 99.999%) within the discharged gas. A paper detailing the full methodology and results of this study can be found here.

Did You Know: about HEPA Filtration?

Whitley H35 Hypoxystation

HEPA, or High Efficiency Particulate Air, is a filtration system which ensures the utmost product protection for your samples.

Unlike competitor systems that draw atmosphere out of the workstation before filtering and reintroducing it, Don Whitley Scientific has created a unique design, which is housed completely inside the workstation. Housing the HEPA system inside the chamber prevents the build-up of moisture which renders HEPA filters ineffective, and cleans the internal atmosphere quickly.

Using innovative circulation technology, all the atmosphere in the chamber passes through the filter 900 times every hour. This quickly creates and maintains an environment exceeding the level of atmospheric cleanliness stipulated by ISO 14644, referenced in the Cell Tissue Culture Directive as the recommended standard for the handling of mammalian cells. We achieve levels of atmospheric cleanliness exceeding the requirements of Class 3 of this standard, which to the best of our knowledge is better than any other manufacturer.

There are currently three Whitley Workstations available with HEPA filtration:

A35 HEPA Anaerobic Workstation
H35 HEPA Hypoxystation
H45 HEPA Hypoxystation.

Proof of the atmospheric cleanliness achieved during the rigorous tests conducted on every HEPA workstation before dispatch is available on request. Please contact us for more information.

This video demonstrates the effectiveness of our system.

NEW Whitley i2 Instrument Workstation

The Whitley i2 Instrument Workstation has been developed in response to a rising number of enquiries from scientists who desire to use Seahorse Extracellular Flux (XF) Analyzers* in hypoxic conditions and were dissatisfied with the solutions available. Rather than adapting an existing chamber for this purpose, Don Whitley Scientific developed a new workstation specifically to suit the precise requirements of Seahorse Bioscience.

Extensive research and careful consideration has gone into the development of the i2, which has resulted in a modular system that includes a number of novel and unique features. This workstation can be used as a stand-alone unit or connected to a Whitley Hypoxystation via the new Whitley Transfer Tunnel, enabling preparation of cell lines under hypoxic conditions and their transfer directly into the i2 without exposure to air.

Another unique feature is the integral incubator, which enables you to precondition cellware and incubate plates and media at 37°C under the same atmospheric conditions as the XF Analyzer.

The i2 maintains an internal temperature no higher than 28°C, excludes carbon dioxide, and provides precise oxygen control.

This workstation, the latest in a long line of well-regarded modified atmosphere workstations from Don Whitley Scientific, is equipped with a generous working area in which to conduct preparatory work and is supplied complete with removable front, bespoke trolley, internal mains sockets, and a wireless footswitch to control the patented oval ports.

Whitley i2 Instrument Workstation connected to a H35 HEPA Hypoxystation
Whitley i2 Instrument Workstation connected to a H35 HEPA Hypoxystation

The 12 litre airlock, with a cycle time of just 60 seconds, accommodates up to 44 × 96 well plates or 84 × T25 tissue culture flasks plus numerous other flasks, pipettes and laboratory consumables.

The Whitley i2 Instrument Workstation is the first in a new range of larger Whitley Workstations developed to allow customers to use a variety of items of specialised equipment under modified atmospheric conditions.

The combination of a Whitley i2 Instrument Workstation and Seahorse XF Analyzer permits simultaneous, real-time analysis of mitochondrial respiration and glycolysis in mammalian cells under precisely controlled hypoxic conditions.

If your research requires unsurpassed accuracy, reproducibility and reliability, then insist on a Whitley Workstation.

 

 

* Not presently suitable for the recently announced Seahorse Bioscience XF-p

H35 HEPA Installed at Liverpool University

Earlier this month, we installed a Whitley H35 HEPA Hypoxystation in the Molecular and Clinical Pharmacology department at the University of Liverpool.

Here’s a picture of Dr. Michael Cross participating in the workstation user-training session.

HEPA Filtered Hypoxystation for New Year Launch

Whitley H35 HEPA Hypoxystation

If you need to work in a HEPA filtered workstation environment – with precise control of oxygen, carbon dioxide, temperature and humidity, for example in stem cell research – this Hypoxystation is for you.

A new Hypoxystation complete with a HEPA filtration system is due to launch in the New Year. Early test results have indicated that the Whitley H35 HEPA Hypoxystation will achieve Class 2 conditions as per the redefined ISO standards for particulate count and classification for air cleanliness – this will be independently verified in due course.

The H35 HEPA Hypoxystation will look exactly like the current H35 but will be deeper by 90mm. The dimensions will therefore be 1255 x 810 x 710mm (w x d x h). This product will have a removable front as standard, fitted with two oval, sleeved ports. CO2 monitoring will be included as standard on this product, a saving of over £2,000.

Options/accessories, literature and specification details will be published on www.meintrup-dws.de in January 2014.

For further information please contact your sales representative or email us on sales@meintrup-dws.de