Whitley H35 Hypoxystation used in heart regeneration project

H35 Australia 1

Dr. Vaibhao Janbandhu is a Postdoctoral Research Scientist at the Victor Chang Cardiac Research Institute (VCCR) in Sydney. He has been in contact with Don Whitley Scientific to explain how his lab’s work has benefited from the use of a Whitley H35 Hypoxystation. Vaibhao uses the Hypoxystation to isolate, culture and characterise adult cardiac stem cells (CSCs).


Dr. Janbandhu had already been using a H35 that was set up at the institute for almost three years before he got his own unit installed last year. Specifically, his project is to find new ways to stimulate heart regeneration during ageing and after heart attack. For this he needs a way to isolate, culture and characterise adult CSCs. In Vaibhao’s words the H35 Hypoxystation seems well suited for this application: “the DWS Hypoxystation provides a highly stabilised HEPA-filtered environment in which levels of oxygen, carbon dioxide, temperature and humidity are precisely controlled and it will be an integral part of the project to advance the project aims”.


Vaibhao explains that mammalian stem cells reside in a specialised cellular microenvironment. This niche and the stem cell niche is characterised by a low partial oxygen pressure. This hypoxic niche protects stem cells from deleterious effects of O2 on proteins and DNA. These precise conditions are essential for Dr. Janbandhu’s work as they accurately replicate the in vivo environment.


His work also see benefits when comparing the use of a Hypoxystation as opposed to using a CO2 incubator. Typically, cell culture work involves methods which include isolating cells under their usual physiologically relevant conditions and then working with them in “bench-top conditions” where cells are exposed to non-physiological oxygen. This can then lead to altered hypoxic response, metabolism, reactive oxygen species and DNA damage response. This metabolic stress introduces unknown outcomes and may lead to results inconsistent with physiological processes. Therefore, the precise control of oxygen levels in cell culture has been shown to be vital for reproducible and physiologically relevant results, transforming the working environment in Vaibhao’s lab.


As well as the precise controlling of conditions, Vaibhao likes the remote access feature on his H35 Hypoxystation. The remote access allows Vaibhao to log into his Hypoxystation’s touchscreen control whilst away from the unit, offering increased flexibility in his working methods. Additionally, he likes how he can view operating conditions, set parameters and change access levels remotely.


Dr. Janbandhu opted to purchase a Hypoxystation from Don Whitley Scientific for the level of service and specification we were able to provide in Australia. Other companies either couldn’t fulfil configuration requirements and didn’t provide sales and or service in Australia. Don Whitley Scientific’s office in Australia, provides both fantastic sales and service nationwide. Vaibhao also states that from “discussion with other research groups across the world we felt confident to go for a DWS Hypoxystation”.


Dr. Vaibhao Janbandhu has this to say about Don Whitley Scientific Pty Ltd: “I would like to thank your company personnel at the customer services division in Australia for their excellent support. Your Sales & Service Manager in Australia, Grant Shallcross, took care of all my queries in a jiffy!”. Vaibhao added that the funding for the purchase came from the James N Kirby Foundation and the Victor Chang Cardiac Research Institute.


H35 Australia 2

Working with Whitley Workstations


Don Whitley Scientific has always strived to be the most innovative manufacturer of scientific equipment. Our leading range of workstations provides some of the most sophisticated options to the microbiology and cell culture industries.

One of the more specific markets Don Whitley Scientific provides for is those wanting to carry out experiments under hypoxic conditions. Researchers may only have the use of an incubator to keep specimens in hypoxic conditions, however this can cause problems when the conditions of the incubator become interrupted, usually when the incubator door is opened to add or remove samples and equipment.

When incubators just won’t do

In a testimonial for Don Whitley Scientific, Brad Wouters from Toronto, Canada shared this issue: “An incubator just isn’t suitable for that because whenever the door is opened, the oxygen concentration changes dramatically and the cells will go through waves of deoxygenation and oxygenation.” An issue like this could then lead to inaccurate results and potentially jeopardise experiments. This is why the Whitley Hypoxystation is ideal for these situations.

Working in a Whitley Workstation

Professor Mann states in his testimonial, “The design of this workstation has also allowed us to use other essential equipment inside the chamber, such as oxygen meters and micromanipulators”. As opposed to an incubator where samples are placed and perhaps left for days without interaction, Whitley Workstations are designed specifically for scientists wanting to carry out tasks inside the workstation.  Optional features on Whitley Workstations allow them to be tailored perfectly to those who want to conduct experiments in their workstation using additional specialised equipment, for example, the removable front option.

Australian testimonial

Maintaining an outstanding international reputation, Don Whitley Scientific exports its range of products to all corners of the globe. In Australia, at the Victor Chang Cardiac Research Institute, the aim is “to reduce the incidence, severity and impact of heart diseases” and for Dr Dunn and her team, precise control of oxygen, carbon dioxide, temperature and humidity is vital. She mentions in her testimonial that with a H35 Hypoxystation she achieves “very accurate and reproducible results”. As well as providing fantastic equipment, Don Whitley Scientific ensures that the advice and service we provide to customers is of a high level, which is also noticed by Dr Dunn, who states, “Don Whitley Scientific made great efforts to ensure our purchase, installation and ongoing use of the machine has been hassle free.”


Dr Dunn stood next to her H35 Hypoxystation
Dr Dunn with her H35 Hypoxystation


Testimonials wanted

If you are working with any equipment from Don Whitley Scientific and would like to let us know about your work and your overall experience with our products, please don’t hesitate to get in touch via our contact form, Facebook or Twitter. We now feature a variety of customer testimonials on our product pages and these can be accessed through the “testimonials” tab (if we have a testimonial for that product).

Click here for Brad Wouters’ testimonial in full

Click here for Professor Mann’s testimonial in full

Click here for Dr Dunn’s testimonial in full

Introducing Easy O2 Calibration

The new, fully automated oxygen calibration system that is currently available on the Whitley H135 Hypoxystation could really make a difference. This system allows you to calibrate your workstation efficiently and easily and so improve the accuracy of experimental data.

The Whitley Automated Calibration System (patent pending) is very user friendly – all you have to do is touch a couple of icons on the colour touchscreen and the routine activates. No further user intervention is required. It  takes just eight minutes to complete and readings are taken at two points – 0% oxygen and 20.9% oxygen levels. You don’t have to enter the chamber at all.

The oxygen sensor is not removed for calibration so the sensor remains within a stable environment and is not subjected to any changes in humidity and temperature when a calibration routine is conducted. Leaving the sensor in place prevents the risk of condensation forming on the component, which could affect its sensitivity, accuracy and life.

You can set the system to prompt you when the next calibration is due (from 7 – 21 days). Don Whitley Scientific recommends that calibration is conducted every two weeks. Full training, confirming how easy the routine is to perform, is provided during the workstation installation procedure. Click on the image below for a video demonstration.






Don Whitley Scientific exhibit at TCES 2015

Don Whitley scientific exhibited at TCES 2015, this annual Tissue and Cell Engineering Society event is held at the University Of Southampton between the 19th-21st of July. TCES 2015 aims to bring together outstanding national and global speakers from stem cell, tissue engineering and regenerative medicine industries, exploring how our understanding of tissue development and the resulting generation of specifiable tissues can be enhanced through stem cell science and regenerative medicine.

With special focus on “Harnessing and translating interdisciplinary research for cell and tissue engineering” the meeting features a programme of workshops and speakers in an effort to justify the excitement expressed from the public and research communities towards the affect ageing and or trauma can have on disease and the loss of tissue. Representatives from Don Whitley Scientific are attending the meeting, pictured is Daniel Secker exhibiting a Whitley H35 Hypoxystation.

Whitley Colour Touchscreens

Don Whitley ‘A’ and ‘H’ Workstations now feature modern, easy to use, colour touch screen interfaces. These colour touchscreens replace the gauges, dials and switches of workstations of the past and are a fitting match for the advanced scientific instruments that Don Whitley Scientific provide.  All options and settings you would expect to access on your Don Whitley workstation feature on these unique, clear displays.

• Set password protected user levels to control what can be done and by whom on the workstation

• Complete a range of functions such as O2 calibration and humidity

• Control mains power, internal lights and set audible alarms

• A generous screen size of 7”, making controlled parameters easily visible

• Download  temperature, humidity and chamber pressure data in seconds via USB

• Unique to Don Whitley workstations

Stem Cell Research in Stockholm


Stem cell researchers from all over the world are currently convening in Stockholm for the annual meeting of the International Society for Stem Cell Research (ISSCR). Topics covered in the event range from pluripotency and differentiation through regeneration to disease modelling and tissue engineering. All aspects of reprogramming and stem cell maintenance on the one hand, and differentiation on the other hand, are intricately influenced by the cellular microenvironment. Oxygenation is a crucial parameter throughout all these processes, both in the natural stem cell niche and during culture in the lab. Low oxygen promotes efficient expansion of stem cells in culture while supporting maintenance of the stem cell phenotype. Hypoxia can increase the proliferation rate and inhibit senescence of stem cells, and promote healing directly and through paracrine effects. Therapies utilizing hypoxic cells exhibit improved homing and engraftment to the target tissues as compared to normoxia. Hypoxia is a major determinant of many diverse aspects of stem cell biology.
Don Whitley Scientific will be at the event exhibiting their largest cell culture workstation to date – the Whitley H135 Hypoxystation.

Hypoxia and stem cells

Later this month, the international stem cell research community will be travelling to Stockholm for the upcoming International Society for Stem Cell Research (ISSCR) meeting covering diverse topics from reprogramming and pluripotency of stem cells through tissue engineering and organ regeneration to therapy with stem cells.

Whitley H135 Hypoxystation
Whitley H135 Hypoxystation

Hypoxia is a crucial parameter determining the fate and development of stem cells, which leads Don Whitley Scientific to exhibit the Hypoxystation controlled environment workstation for low oxygen cell culture (see us on stand no. B15:33). Dr. Burga Kalz Fuller, Product Manager at our American distributor, HypOxygen, has summarized five recent papers delineating the role of hypoxia in stem cell research:


Free T-Shirt: Hypoxia Research


Do you culture cells in hypoxic conditions? For a limited time only, you can earn a free t-shirt by taking a few minutes to answer our questions and help us to better fulfil your cell culture needs.

We want to know what your research focus is, how you conduct your cell culture and how you think oxygen content might influence aspects of your cell culture.

Please click here to complete a very short online form.

Hypoxia and Cancer Metabolism

An ever-increasing number of scientific papers are being produced that highlight the effects and importance of hypoxia in various research fields. Dr Burga Kalz Fuller, Product Manager at our American distributor, HypOxygen, has summarised five interesting and recent papers concerning hypoxia and its role in cancer metabolism research:

“Hypoxia signaling pathways in cancer metabolism: the importance of co-selecting interconnected physiological pathways” Masson & Ratcliffe, 2014.

In this paper, the role of hypoxia – as mediated by HIF, interfacing with many different oncogenic pathways – is reviewed. Activated HIF both suppresses mitochondrial metabolism and up-regulates biosynthesis of intermediates needed by proliferating cells in cancer. Complex relationships between HIF activation and cancer phenotype are discussed in detail by these Oxford UK researchers.



The Fastest Oxygen Control on the Market

If you need a workstation that responds rapidly to changes in oxygen set point, read on. We have conducted tests that lead us to believe that no other manufacturer’s hypoxic workstations are able to offer the same accuracy and speed of response to set point changes as those achievable in Whitley Hypoxystations.