Whitley H35 Hypoxystation used in heart regeneration project

H35 Australia 1

Dr. Vaibhao Janbandhu is a Postdoctoral Research Scientist at the Victor Chang Cardiac Research Institute (VCCR) in Sydney. He has been in contact with Don Whitley Scientific to explain how his lab’s work has benefited from the use of a Whitley H35 Hypoxystation. Vaibhao uses the Hypoxystation to isolate, culture and characterise adult cardiac stem cells (CSCs).

 

Dr. Janbandhu had already been using a H35 that was set up at the institute for almost three years before he got his own unit installed last year. Specifically, his project is to find new ways to stimulate heart regeneration during ageing and after heart attack. For this he needs a way to isolate, culture and characterise adult CSCs. In Vaibhao’s words the H35 Hypoxystation seems well suited for this application: “the DWS Hypoxystation provides a highly stabilised HEPA-filtered environment in which levels of oxygen, carbon dioxide, temperature and humidity are precisely controlled and it will be an integral part of the project to advance the project aims”.

 

Vaibhao explains that mammalian stem cells reside in a specialised cellular microenvironment. This niche and the stem cell niche is characterised by a low partial oxygen pressure. This hypoxic niche protects stem cells from deleterious effects of O2 on proteins and DNA. These precise conditions are essential for Dr. Janbandhu’s work as they accurately replicate the in vivo environment.

 

His work also see benefits when comparing the use of a Hypoxystation as opposed to using a CO2 incubator. Typically, cell culture work involves methods which include isolating cells under their usual physiologically relevant conditions and then working with them in “bench-top conditions” where cells are exposed to non-physiological oxygen. This can then lead to altered hypoxic response, metabolism, reactive oxygen species and DNA damage response. This metabolic stress introduces unknown outcomes and may lead to results inconsistent with physiological processes. Therefore, the precise control of oxygen levels in cell culture has been shown to be vital for reproducible and physiologically relevant results, transforming the working environment in Vaibhao’s lab.

 

As well as the precise controlling of conditions, Vaibhao likes the remote access feature on his H35 Hypoxystation. The remote access allows Vaibhao to log into his Hypoxystation’s touchscreen control whilst away from the unit, offering increased flexibility in his working methods. Additionally, he likes how he can view operating conditions, set parameters and change access levels remotely.

 

Dr. Janbandhu opted to purchase a Hypoxystation from Don Whitley Scientific for the level of service and specification we were able to provide in Australia. Other companies either couldn’t fulfil configuration requirements and didn’t provide sales and or service in Australia. Don Whitley Scientific’s office in Australia, provides both fantastic sales and service nationwide. Vaibhao also states that from “discussion with other research groups across the world we felt confident to go for a DWS Hypoxystation”.

 

Dr. Vaibhao Janbandhu has this to say about Don Whitley Scientific Pty Ltd: “I would like to thank your company personnel at the customer services division in Australia for their excellent support. Your Sales & Service Manager in Australia, Grant Shallcross, took care of all my queries in a jiffy!”. Vaibhao added that the funding for the purchase came from the James N Kirby Foundation and the Victor Chang Cardiac Research Institute.

 

H35 Australia 2

Stem Cell Research in Stockholm

 

Stem cell researchers from all over the world are currently convening in Stockholm for the annual meeting of the International Society for Stem Cell Research (ISSCR). Topics covered in the event range from pluripotency and differentiation through regeneration to disease modelling and tissue engineering. All aspects of reprogramming and stem cell maintenance on the one hand, and differentiation on the other hand, are intricately influenced by the cellular microenvironment. Oxygenation is a crucial parameter throughout all these processes, both in the natural stem cell niche and during culture in the lab. Low oxygen promotes efficient expansion of stem cells in culture while supporting maintenance of the stem cell phenotype. Hypoxia can increase the proliferation rate and inhibit senescence of stem cells, and promote healing directly and through paracrine effects. Therapies utilizing hypoxic cells exhibit improved homing and engraftment to the target tissues as compared to normoxia. Hypoxia is a major determinant of many diverse aspects of stem cell biology.
Don Whitley Scientific will be at the event exhibiting their largest cell culture workstation to date – the Whitley H135 Hypoxystation.

Hypoxia and stem cells

Later this month, the international stem cell research community will be travelling to Stockholm for the upcoming International Society for Stem Cell Research (ISSCR) meeting covering diverse topics from reprogramming and pluripotency of stem cells through tissue engineering and organ regeneration to therapy with stem cells.

Whitley H135 Hypoxystation
Whitley H135 Hypoxystation

Hypoxia is a crucial parameter determining the fate and development of stem cells, which leads Don Whitley Scientific to exhibit the Hypoxystation controlled environment workstation for low oxygen cell culture (see us on stand no. B15:33). Dr. Burga Kalz Fuller, Product Manager at our American distributor, HypOxygen, has summarized five recent papers delineating the role of hypoxia in stem cell research:

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HEPA Filtered Hypoxystation for New Year Launch

Whitley H35 HEPA Hypoxystation

If you need to work in a HEPA filtered workstation environment – with precise control of oxygen, carbon dioxide, temperature and humidity, for example in stem cell research – this Hypoxystation is for you.

A new Hypoxystation complete with a HEPA filtration system is due to launch in the New Year. Early test results have indicated that the Whitley H35 HEPA Hypoxystation will achieve Class 2 conditions as per the redefined ISO standards for particulate count and classification for air cleanliness – this will be independently verified in due course.

The H35 HEPA Hypoxystation will look exactly like the current H35 but will be deeper by 90mm. The dimensions will therefore be 1255 x 810 x 710mm (w x d x h). This product will have a removable front as standard, fitted with two oval, sleeved ports. CO2 monitoring will be included as standard on this product, a saving of over £2,000.

Options/accessories, literature and specification details will be published on www.meintrup-dws.de in January 2014.

For further information please contact your sales representative or email us on sales@meintrup-dws.de