New Poster on Gynaecological Cancer Cell Metabolism

On the 1st and 2nd of December, Don Whitley Scientific’s Sales Director, Steve Robertson, will be attending the Molecular Medicine Workshop (Red Temática de investigación cooperativa en cáncer) in Seville, Spain, with Dr. Ayse Latif from the University of Manchester. Dr Latif will present a poster detailing her recent work: Interrogating gynaecological cancer cell metabolism at different oxygen tensions: Results using a novel modified atmosphere cellular handling system.

Whitley i2 Instrument Workstation
Whitley i2 Instrument Workstation

Dr Latif used a Seahorse Bioscience Extracellular Flux Analyzer in her research, and the Whitley i2 Instrument Workstation has played a significant role in her findings by allowing the Analyzer to be used in a controlled hypoxic environment.


If you would like to know more about the i2 Workstation, please contact us on +44 (0)1274 595728 or email



Did You Know: about Quality Counts?

Whitley Automated Spiral Plater (WASP)

When you purchase a WASP (Whitley Automated Spiral Plater), you have the option to join our Quality Counts scheme. Free of charge for the first 12 months, this programme provides valuable assurance that your WASP is functioning correctly and that samples are being analysed accurately.

Subscribers to the scheme will receive a sample on a monthly basis. Each customer simply spiral plates the sample and then sends the result back to our laboratory. Don Whitley Scientific will then collate the results and advise as to whether or not you are within specification. Should your results fall outside acceptable limits, we will offer advice, training or service facilities as appropriate.

If you choose to continue your subscription after the first year, discounts are available for organisations wishing to cover more than one WASP with the scheme.

Please contact us for more information.

Please note: This scheme is only available to customers in the UK and Eire.


NEW Whitley i2 Instrument Workstation

The Whitley i2 Instrument Workstation has been developed in response to a rising number of enquiries from scientists who desire to use Seahorse Extracellular Flux (XF) Analyzers* in hypoxic conditions and were dissatisfied with the solutions available. Rather than adapting an existing chamber for this purpose, Don Whitley Scientific developed a new workstation specifically to suit the precise requirements of Seahorse Bioscience.

Extensive research and careful consideration has gone into the development of the i2, which has resulted in a modular system that includes a number of novel and unique features. This workstation can be used as a stand-alone unit or connected to a Whitley Hypoxystation via the new Whitley Transfer Tunnel, enabling preparation of cell lines under hypoxic conditions and their transfer directly into the i2 without exposure to air.

Another unique feature is the integral incubator, which enables you to precondition cellware and incubate plates and media at 37°C under the same atmospheric conditions as the XF Analyzer.

The i2 maintains an internal temperature no higher than 28°C, excludes carbon dioxide, and provides precise oxygen control.

This workstation, the latest in a long line of well-regarded modified atmosphere workstations from Don Whitley Scientific, is equipped with a generous working area in which to conduct preparatory work and is supplied complete with removable front, bespoke trolley, internal mains sockets, and a wireless footswitch to control the patented oval ports.

Whitley i2 Instrument Workstation connected to a H35 HEPA Hypoxystation
Whitley i2 Instrument Workstation connected to a H35 HEPA Hypoxystation

The 12 litre airlock, with a cycle time of just 60 seconds, accommodates up to 44 × 96 well plates or 84 × T25 tissue culture flasks plus numerous other flasks, pipettes and laboratory consumables.

The Whitley i2 Instrument Workstation is the first in a new range of larger Whitley Workstations developed to allow customers to use a variety of items of specialised equipment under modified atmospheric conditions.

The combination of a Whitley i2 Instrument Workstation and Seahorse XF Analyzer permits simultaneous, real-time analysis of mitochondrial respiration and glycolysis in mammalian cells under precisely controlled hypoxic conditions.

If your research requires unsurpassed accuracy, reproducibility and reliability, then insist on a Whitley Workstation.



* Not presently suitable for the recently announced Seahorse Bioscience XF-p

Cycling Challenge Raises £430,000

A huge ‘Well done’ to Judith Walton who recently returned from undertaking a gruelling cycling challenge in Tanzania and Zanzibar. She was part of a team of 80 women who undertook the challenge to raise money for Genesis Research Trust – and the group as a whole (140 people) managed to raise a massive £430,000.

Judith’s journal of her time there describes how they cycled up to 103km (that’s 64 miles) a day in temperatures of over 35°C and 86% humidity on sandy, uneven roads. She mentions at one point, “Some smells along the way have been fantastic and some I never want to smell again.” The group took with them supplies of chalks, pens, pencils, toothbrushes and sweets. These gifts were delivered to schools along their route and handed out to local children, who though poor and barefoot, were a constant source of encouragement to the cyclists.


At the end of the trip Judith explains how all the exhausted cyclists gathered together on the beach at sunset to light a candle and reflect in silence on the reasons why they were all undertaking this gigantic personal challenge. Then they each floated a flower to represent their individual thoughts. Judith comments: “The atmosphere was electric. I have never been part of anything so moving in my life …”

Genesis Research Trust raises money for the largest UK-based collection of scientists and clinicians who are researching the causes and cures for conditions that affect the health of women and babies.

Judith’s next challenge for the Genesis Research Trust is a 1,000 mile cycle ride from John O’Groats to Lands End (JOGLE) in May 2015. For anyone wishing to sponsor Judith, this is a link to her ‘Just Giving’ page and any further donations will go to JOGLE.


New Software for ProtoCOL 3

Don Whitley Scientific is pleased to introduce a revolutionary new software module for the ProtoCOL 3 automated colony counter. The addition of this unique software, makes ProtoCOL 3 the world’s first commercial automatic microbial identification and counter of colonies cultured on CHROMagar plates and means that with minimal training, microbiologists can use the system  to rapidly identify and enumerate all key clinical, water and food-borne pathogens.

The new ProtoCOL 3 software module was developed by Synbiosis, world-leading manufacturer of automated microbiological systems, and major media manufacturer CHROMagar. This module ensures that the ProtoCOL 3 system can accurately identify any bacteria or yeast cultured on a CHROMagar plate in less than a minute, saving microbiologists hours of visually inspecting colonies, and manually recording results.

The ProtoCOL 3 system performs these ground-breaking tasks by utilising unique, patented red, blue and green lighting to capture a life-like colour image of the colonies on the plates. The new software module analyses the image and is so sophisticated that it can distinguish between rose pink and dusty pink, as well as turquoise from steel blue. This allows precise identification of common pathogens, including Salmonella spp., Staphylococcus aureus, Candida albicans, E.coli, Group B Streptococci, Listeria spp., Vibrio spp and Pseudomonas spp. The system also simultaneously enumerates the different coloured colonies of each species, providing objective, consistent data and reducing operator errors, to generate accurate results which can be stored as images and Excel spreadsheets.

Kate George, Applications Specialist at Synbiosis explained: “When manually processing large numbers of plates, microbiologists find identifying and counting microbes time-consuming, repetitive tasks and have often requested a system that can automate these. Accurately identifying microbial pathogens, as well as enumerating so many types of colonies on different agars is difficult. However, we have worked extensively for several years with CHROMagar and are delighted with the resulting powerful analytical software because it makes the ProtoCOL 3 the only commercial system that can perform this seemingly impossible rapid microbial identification task.”

Kate added: “Clinical microbiologists, as well as food, beverage and water quality control scientists looking to obtain precise microbial identification results and increase their colony counting throughput can now enjoy these benefits by installing a ProtoCOL 3 with its revolutionary rapid identification software in their laboratories.”


H35 HEPA Installed at Liverpool University

Earlier this month, we installed a Whitley H35 HEPA Hypoxystation in the Molecular and Clinical Pharmacology department at the University of Liverpool.

Here’s a picture of Dr. Michael Cross participating in the workstation user-training session.

Distributor Sales Training

Hilde, Linda, Marcel - Sales Nov 2014

Last week saw the latest Don Whitley Scientific Distributor Sales Training session at the Shipley, UK head office. Delegates attended from three of our European distributors – VWR (Norway), Meintrup DWS (Germany) and Labconsult (Belgium). The programme included sales presentations, comprehensive product demonstrations and Q&A sessions with product and sales specialists. There was also the opportunity to discuss new developments with the R&D department, receive top tips from our experienced sales team and discuss the latest information regarding other products in the market place. (more…)