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Whitley H35 Hypoxystation

Die Hypoxysation ist das ideale Produkt für die Zellkulturforschung. Unsere neuen Arbeitsstationen bilden vergeichbare in vivo Bedingungen nach.

Die Whitley H35 bietet Ihnen folgende Möglichkeiten:

  • O2-Kontrolle in 0,1 %-Schritten, von 0,1 % bis 20 %

  • unkomplizierte Kalibrierung der Gassensoren

  • Einfache und präzise Temperaturkontrolle

  • Kontrolle der relativen Feuchtigkeit bis 80 %

Hauptmerkmale

  • Touch Screen- Schnittstelle:

    - ermöglicht gleichzeitige einfache Überwachung aller Parameter
    - andere Skalen, Schalter oder Messgeräte sind überflüssig

  • Die integrierte Schleuse sorgt für schnellstmöglichen Transfer von Zellwaren in die und aus der Arbeitsstation, Dauer des Schleusenzyklus 1 Min.
     
  • Patentierte ergonomische Handeingänge ermöglichen das Arbeiten mit Handschuhen und mit bloßen Händen
     
  • jedes Gerät wird nach Ihrem bestimmten Forschungsgebiet produziert


Optionen

  • CO2 Monitoring: in 0,1 %-Schritten, von 0,1 % bis 15 %

  • Abnehmbare Frontscheibe

  • Vakuumentnahmestelle

  • 15 cm Einplatteneinschub - ‚Letterbox‘

  • Befeuchtungssystem

  • Interne Doppelsteckdose

  • Probenentnahmestelle

  • Datenlogger

  • Oxygenprofiling

  • Trolley

  • Regale

  • Automatisches Stulpenbegasungsystem

Kapazität: 300 Liter

Weitere Informationen zu unserer Whitley H35 finden Sie unter www.hypoxystation.de

Spezifikationen ansehen

Spezifikation

Kapazität (Liter) 300 Liter
Schleusengröße 12 Liter / beherbergt bis zu 44 x 96 Wellplatten or 84 x T25 Kulturflaschen or 8 x T175 Kulturflaschen
Handeingangssystem Manuell
Gaszufuhr CO2/Air/N2
Fußschalter kabellos
Automatisches Stulpenbegasungssystem Option erhältlich
Interne Steckdose Option erhältlich
Untersuchungslampe Option erhältlich
Einplatteneinschub Option erhältlich
Sauerstoffüberwachungssystem keine Angabe
Kühleinheit keine Angabe
Datenüberwachungssystem Option erhältlich
Schleusenzyklusdauer (Sekunden) 60
zusätzliche druckdichte Kabeleinführung Option erhältlich
Mikroskop Option erhältlich
automatisches Entfeuchtungssystem Standardmäßig eingebaut
automatisches Befeuchtungssystem Option erhältlich
Abmessungen (B x T x H – mm) 1255 / 720 / 710
Gewicht (lbs/kg) 231 / 105
CO2-Monitoring Standardmäßig eingebaut
Vakuumentnahmestelle Option erhältlich
Temperaturbereich (°C) 5°C über Umgebung bis zu 45°C
Touchscreensteuerung Ja

Bilder

Video

Downloads

Literatur

H35 Hypoxystation Installationsanforderungen_englischWhitley H35 Hypoxystation Fact SheetWhitley Hypoxystation Range BrochureGas consumption in a Whitley Hypoxystation is lower than other hypoxia workstations ...

Veröffentlicht Papiere Poster

Stegeman H et al 2012 - Activation of AKT by hypoxia: a potential target for hypoxic tumors of the head and neckMuth M, et al. Hypoxia-induced down-regulation of microRNA-449a/b impairs control over targeted SERPINE1 (PAI-1) mRNA - a mechanism involved in SERPINE1 (PAI-1) overexpression. CORRECTION. Journal of Translational Medicine 2011, 9:24Richards R & See V. Brain Tumour Cells Can Survive and Thrive in the Hypoxic Solid Tumour MicroenvironmentWennemers M, et al TRIB3 protein denotes a good prognosis in breast cancer patients and is associated with hypoxia sensitivity. Radio Therapy and Oncology, Vol 101, Issue 1, Oct 2011, 198-202Ortiz-Barahona A, et al. Genome-wide identification of hypoxia-inducible factor binding sites and target genes by a probabilistic model integrating transcription-profiling data and in silico binding site prediction. Nucleic Acids Research, 2010, Vol Pescador N, et al. Hypoxia promotes glycogen accumulation through Hypoxia Inducible Factor (HIF)-medicated induction of glycogen synthase 1. Institute of Genetics and Molecular and Cellular Biology. March 2010, Vol 5, Issue 3Tiana M, et al. A role for insulator elements in the regulation of gene expression response to hypoxia. Nucleic Acids Research 2011, 1-12Li L, et al. Rnf8 deficiency impairs class switch recombination, spermatogenesis, and genomic integrity and predisposes for cancer. The Journal of Experimental Medicine, Vol 207, No 5, April 12, 2010, 983-997Ahmadi M, et al. Hypoxia modulates the activity of a series of clinically approved tyrosine kinase inhibitorsvan de Schootbrugge C et al, Effect of hypoxia on the expression of αB-crystallin in head and neck squamous cell carcinomaMacpherson T et al, Physiological intestinal oxygen modulates the Caco-2 cell model and increases sensitivity to the phytocannabinoid cannabidiolXiang B et al, Expression of oxidored nitro domain-containing protein 1(NOR1) impairs nasopharyngeal carcinoma cells adaptation to hypoxia and inhibits PDK1 expressionMiranda et al, June 2013: A Cyclic Peptide Inhibitor of HIF‑1 Heterodimerization That Inhibits Hypoxia Signaling in Cancer CellsAsby et al, July 2014: HIF-1 promotes the expression of its a-subunit via an epigenetically regulated transactivation loopHerrmann et al, 2015 - Cellular memory of hypoxia elicits neuroblastoma metastasis and enables invasion by non-aggressive neighbouring cellsCojocari D et al. Autophagy promotes tolerance to hypoxia through maintenance of metabolic homeostasis 2015Koch E et al. Hypoxia promotes stemness and poor prognosis through epigenetic regulation of DICER 2015De Bruin et al. Genome-wide analysis reveals NRP1 as a direct HIF1a-E2F7 target in the regulation of motorneuron guidance in vivoRichards R et al. Cell Cycle Progression in Gioblastoma. Mar 2016 peerj-1755Weiss J T et al. Palladium-mediated dealkylation of N-propargyl-floxuridine as a bioorthogonal oxygen-independent prodrug strategy. 2015Burrows N et al. Hypoxia-induced nitric oxide production and tumour perfusion is inhibited by pegylated arginine deiminase. nature.com. Scientific Reports 6, article no. 22950. 2016Leszczynska K B et al. Mechanisms and consequences of ATMIN repression in hypoxic conditions: roles for p53 and HIF-1. nature.com. Scientific Reports 6, article no. 21698. 2016Jiang Y et al. Hypoxia Potentiates the Radiation-Sensitizing Effect of Olaparib in Human Non-Small Cell Lung Cancer Xenografts by Contextual Synthetic Lethality. International Journal of Radiation Oncology. 2016Sandt C et al. Use of infrared microspectroscopy to elucidate a specific chemical signature associated with hypoxia levels found in glioblastoma. Royal Society of Chemistry. Analyst. 2016. 141.870Palazon A, et al. The HIF-1α Hypoxia Response in Tumor-Infiltrating T Lymphocytes Induces Functional CD137 (4-1BB) for Immunotherapy. Cancer Discovery, June 2012Labiano S et al. Hypoxia-induced soluble CD137 in malignant cells blocks CD137L-costimulation as an immune escape mechanism. Oncoimmunology 2016, Vol5, No1 1-10Vaapil M et al. 2012. Hypoxic conditions induce a cancer-like phenotype in human breast epithelial cells. PLoS ONE 7(9):e 46543. doi:10.1371/journal.pone.0046543Timpano and Uniacke. 2016. Human Cells Cultured Under Physiological Oxygen Utilize Two Cap-binding Proteins to Recruit Distinct mRNAs for Translation. Journal of Biological Chemistry 291(20):jbc.M116.717363Taylor SE, Bagnall J, Mason D, Levy R, Fernig DG, See V. 2016 Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility. Open Biol. 6: 160195. http://dx.doi.org/10.1098/rsob.160195Shammout B et Al. BES Poster. H35Elkashef S M et al. 2016. Polysialic acid sustains cancer cell survival and migratory capacity in a hypoxic environment. Nature Scientific Reports 2016 6:33026Campbell E J et al. A scorbate availability affects tumor transplantation-take rate and increases tumor rejection in Gulo-/-mice. Hypoxia 2016:4 41-52Mohlin S et al. PI3K-mTORC2 but not PI3K-mTORC1 regulates transcription of HIF2A/EPAS1 and vascularization in neuroblastoma. Cancer Res 2015; 75(21); 4617-28Mohlin S et al. HIF2A and IGF2 expression correlates in human neuroblastoma cells and normal immature sympathetic neuroblasts. Neoplasia 2013 Vol 15, No 3, 328-34Tan Q et al. Role of Autophagy as a Survival Mechanism for Hypoxic Cells in Tumors. Neoplasia (2016) 18, 347-355Li R et al. Rnf8 deficiency impairs class switch recombination, spermatogenesis, and genomic integrity and predisposes for cancer. J. Exp. Mel. Vol 207 No 5, 983-997. Doi:10.1084/jem.20092437Alam et al. 2016. HIF2α contributes to antiestrogen resistance via positive bilateralBremm A et al. 2014. Cezanne (OTUD 7 B) regulates HIF-1a homeostasis in a proteasome-independent manner

Artikel

Time Lapse Microscopy in a Whitley WorkstationThe Fastest O2 Control on the Market

Anwendungshinweise

Application Note_1: Interview with Biplab DasguptaApplication Note_4: Interview with Houda BenlhabibApplication Note_3: Interview with Margaret Petroff

Andere

Optionen Einplatteneinschub_englisch

Referenzen

i2 and H35 HEPA Workstations at ICR, London

Datum: 2015-04-01

Whitley i2 Instrument Workstation used in deciphering the metabolic properties of Breast Cancer The Institute of Cancer Research, London is...

H35 Hypoxystation-3

Datum: 2012-08-17

Accurate control of the oxygen tension to which cells are exposed to has become an important consideration in many aspects of biomedical research....

H35 Hypoxystation-2

Datum: 2012-08-01

One of our Whitley H35 Hypoxystation customers comments on his use of the workstation and explains how it is helping in his...

H35 Hypoxystation-1

Datum: 2012-07-26

The Importance of Oxygen Control in Cancer Research Dr Roger Phillips, at the University of Bradford’s Institute of Cancer Therapeutics,...